9027-67-2

Basic information

• Product Name: TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE
• Synonyms: EC 2.7.7.31;TDT;TDTASE;TDT ENZYME;TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE (TDT);TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE;TERMINAL TRANSFERASE;NUCLEOSIDE TRIPHOSPHATE:DNA DEOXYNUCLEOTIDYL-EXOTRANSFERASE
• CAS NO: 9027-67-2
• Molecular Formula: C4H10S3
• Molecular Weight: 154.3172
• EINECS: 232-819-6
• Product Categories: enzyme
• Mol File: 9027-67-2.mol
• Article Data: 0

Chemical Properties

• Appearance: /buffered aqueous glycerol solution
• Storage Temp.: −20°C
• CAS DataBase Reference: TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE(CAS DataBase Reference)
• NIST Chemistry Reference: TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE(9027-67-2)
• EPA Substance Registry System: TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE(9027-67-2)

Safety Data

• Hazard Codes: T
• Statements: 49-52/53
• Safety Statements: 22-24/25-61-45-53
• RIDADR: UN 3316 9
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 9027-67-2(Hazardous Substances Data)

Usage

Description

TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE, also known as TdT, is an enzyme derived from calf thymus and recombinantly produced in E. coli. It plays a crucial role in the TUNEL reaction, which is a technique used for detecting and quantifying apoptotic cell death at the single-cell level in cells and tissues.

Uses

Used in Biomedical Research:
TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE is used as a key enzyme in the TUNEL reaction for detecting apoptosis in situ. It helps label DNA strand breaks, allowing researchers to identify and quantify apoptotic cell death at a cellular level.
Used in Diagnostic Applications:
In the field of diagnostics, TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE is employed as a component of the TUNEL reaction mixture, which is essential for performing the TUNEL assay. This assay is widely used to detect and quantify apoptosis in various cell types and tissues, aiding in the diagnosis and study of diseases and conditions associated with cell death.
Used in Pharmaceutical Development:
TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE can also be utilized in the development of pharmaceuticals targeting apoptosis. By understanding the role of this enzyme in the TUNEL reaction, researchers can develop drugs that modulate apoptosis, potentially leading to treatments for diseases characterized by abnormal cell death or survival.