9029-47-4

Basic information

• Product Name: EC 1.13.11.3
• Synonyms: PROTOCATECHUATE 3,4-DIOXYGENASE;EC 1.13.11.3;protocatechuate 3,4-dioxygenase from*pseudomonas;PROTOCATECHUATE 3,4-DIOXYGENASE FROM*PSE UDOMONAS SP;frompseudomonasspecies,lyophilizedpowderactivity:3-5umgsolidcontainsapprox.40;Protocatechuate-3,4-dioxygenase,from Pserdomonas sp.;Protocatechuate 3,4-Dioxygenase, recombinant from bacteria, solution;rPCO, Protocatechuate: oxygen 3,4-oxidoreductase (decyclizing)
• CAS NO: 9029-47-4
• Molecular Weight: 0
• Product Categories: 1.13.x.x Acting on single donors with incorporation of molecular oxygen;1.x.x.x Oxidoreductases;Application Index;Diagnostic and Analytical EnzymesMetabolomics;Enzymes, Inhibitors, and Substrates;General Metabolic Enzymes;General Metabolic Enzymes I-ZEnzyme Class Index;enzyme
• Mol File: 9029-47-4.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• Storage Temp.: -20°C
• CAS DataBase Reference: EC 1.13.11.3(CAS DataBase Reference)
• NIST Chemistry Reference: EC 1.13.11.3(9029-47-4)
• EPA Substance Registry System: EC 1.13.11.3(9029-47-4)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 9029-47-4(Hazardous Substances Data)

Usage

Description

EC 1.13.11.3 is an enzyme that plays a crucial role in the degradation of aromatic compounds. It employs a nonheme iron center to catalyze the conversion of protocatechuate (PCA) and molecular oxygen into β-carboxy-cis,cis-muconic acid. This enzyme is involved in the oxygen scavenging system, which helps protect against oxidative stress and damage.

Uses

Used in Oxygen Scavenging Systems:
EC 1.13.11.3 is used as an enzyme in oxygen scavenging systems for its ability to convert PCA and molecular oxygen into β-carboxy-cis,cis-muconic acid. This application helps protect against oxidative stress and damage in various settings.
Used in Imaging Buffer Preparation:
In the field of molecular biology, EC 1.13.11.3 is used as a component in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP, and protocatechuate. This imaging buffer is essential for single molecule motility assays, which are crucial for studying the movement and function of molecular motors like dynein.
Used in Antioxidant Systems:
The enzyme EC 1.13.11.3 works in conjunction with the antioxidant Trolox to suppress slow blinking and photobleaching of cyanine dyes. This combination is important for maintaining the stability and integrity of dyes used in various imaging and detection techniques, ensuring accurate results and minimizing artifacts.

Biochem/physiol Actions

Protocatechuate 3,4-Dioxygenase catalyzes the degradation of 3,4-dihydroxybenzoate (protocatechuate) into β-carboxy-cis,cis-muconate.