9032-92-2

Basic information

• Product Name: O-GLYCOSIDASE
• Synonyms: EC 3.2.1.97;ENDO-ALPHA-N-ACETYLGALACTOSAMINIDASE;ENDO-ALPHA-N-ACETYLGALACTOSAMINIDASE, STREPTOCOCCUS PNEUMONIAE;O-GLYCOSIDASE;O-GLYCOPEPTIDE ENDO-D-GALACTOSYL-N-ACETYL-ALPHA-GALACTOSAMINOHYDROLASE;O-GLYCAN PEPTIDE HYDROLASE;O-GLYCOSIDASE, EXPRESSED IN E. COLI;endo-α-n-acetylgalactosaminidase
• CAS NO: 9032-92-2
• Molecular Weight: 0
• EINECS: 232-888-2
• Mol File: 9032-92-2.mol
• Article Data: 0

Chemical Properties

• Appearance: /buffered aqueous solution
• Storage Temp.: 2-8°C
• CAS DataBase Reference: O-GLYCOSIDASE(CAS DataBase Reference)
• NIST Chemistry Reference: O-GLYCOSIDASE(9032-92-2)
• EPA Substance Registry System: O-GLYCOSIDASE(9032-92-2)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 9032-92-2(Hazardous Substances Data)

Usage

Uses

O-Glycosidase is used to release the Gal-β(1,3)-GaINAc unit from O-glycans (glycoproteins or glycopeptides). Bindings to serine as well as to threonine are hydrolyzed. Substituents at the disaccharide (e.g., sialic acid) prevent the hydrolysis and, therefore, have to be removed chemically or enzymatically (e.g., with neuraminidase from Arthrobacter or from Vibrio cholerae).O-Glycosidase has been used to selectively deglycosylated chondroitin sulfate proteoglycans (CSPGs).

General Description

O-Glycan-peptide hydrolaseO-Glycopeptide endo-D-galactosyl-N-acetyl-α-galactosamino hydrolaseO-Glycosidase is isolated from the culture filtrate of Diplococcus pneumoniae

Biochem/physiol Actions

Releases unsubstituted Ser- and Thr-linked β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase..