9067-77-0

Basic information

• Product Name: EC 4.1.1.31
• Synonyms: ORTHOPHOSPHATE: OXALOACETATE CARBOXYLYASE-[PHOSPHORYLATING];PHOSPHOENOLPYRUVATE CARBOXYLASE;PHOSPHOENOLPYRUVATE CARBOXYLASE (MAIZE LEAVES);PHOSPHOENOLPYRUVATE CARBOXYLASE, ZEA MAYS;PEP-C;PEP CARBOXYLASE;EC 4.1.1.31;carboxylase,phosphoenolpyruvate(phosphate)
• CAS NO: 9067-77-0
• Molecular Formula: NULL
• Molecular Weight: 0
• EINECS: 232-958-2
• Mol File: 9067-77-0.mol
• Article Data: 0

Chemical Properties

• Appearance: /ammonium sulfate suspension
• Storage Temp.: 2-8°C
• CAS DataBase Reference: EC 4.1.1.31(CAS DataBase Reference)
• NIST Chemistry Reference: EC 4.1.1.31(9067-77-0)
• EPA Substance Registry System: EC 4.1.1.31(9067-77-0)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 22-24/25-36-26
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 9067-77-0(Hazardous Substances Data)

Usage

Description

EC 4.1.1.31, also known as phosphoenolpyruvate carboxylase (PEPC), is an enzyme that catalyzes the reversible carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), which is a key step in the process of carbon fixation in plants. It plays a crucial role in various metabolic pathways, including photosynthesis, respiration, and gluconeogenesis.
Used in Clinical Analysis:
EC 4.1.1.31 is used as an enzyme for enzymatic determination of carbon dioxide when coupled with malate dehydrogenase. This application is useful in clinical analysis for measuring carbon dioxide levels in biological samples.
Used in Plant Biology Research:
In the field of plant biology, EC 4.1.1.31 is used as a tool to study carbon assimilation, post-translational regulation, and allosteric regulation in various plants. This helps researchers to better understand the mechanisms of carbon fixation and its regulation in plants, which is essential for optimizing plant growth and productivity.
Used in Photosynthesis Studies:
EC 4.1.1.31 is also used in studies related to photosynthesis, as it plays a key role in the process of carbon fixation during the Calvin cycle. Understanding the function and regulation of this enzyme can provide insights into the efficiency of photosynthesis and help develop strategies to improve crop yields.
Used in Respiration and Gluconeogenesis Research:
In addition to its role in photosynthesis, EC 4.1.1.31 is also involved in respiration and gluconeogenesis. It is used in research to study the interplay between these metabolic pathways and their regulation, which can have implications for understanding energy production and utilization in plants and other organisms.

Biochem/physiol Actions

Phosphoenolpyruvate carboxylase (PEPC) catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP) to form the four-carbon compound oxaloacetate and inorganic phosphate . In CAM and C4 plants, PEPC catalyzes the photosynthetic assimilation of CO2 into an organic acid. PEPC is activated by glucose-6-phosphate and is inhibited by malate and aspartate. PEPC comprises about 0.5-2% of the soluble protein in alfalfa and soybean nodules .