93232-29-2Relevant articles and documents
A New Tetradentate β-Diketonate-Europium Chelate That Can Be Covalently Bound to Proteins for Time-Resolved Fluoroimmunoassay
Yuan, Jingli,Matsumoto, Kazuko,Kimura, Hiroko
, p. 596 - 601 (1998)
A new chlorosulfonylated tetradentate β-diketone, 4,4′-bis(1″,1″,1″,2″,2″,3″,3″- heptafluoro-4″,6″-hexanedion-6″-y1)chlorosulfo-o-terphenyl (BHHCT), was synthesized as a chelating label for Eu3+. BHHCT can be covalently bound to proteins under mild conditions and forms a strongly fluorescent chelate with Eu3+. Bovine serum albumin (BSA) and streptavidin (SA) were labeled with BHHCH-Eu3+, and the latter was used for time-resolved fluoroimmunoassay of a-fetoprotein (AFP) in human sera. A remarkably high sensitivity was obtained, with a detection limit of 4.1 × 10-3 pg/mL, which corresponds to an improvement of about 4-5 orders of magnitude, compared to those of all conventional immunoassays including fluoroimmunoassay, enzyme immunoassay, and radioimmunoassay. The high sensitivity has been attained both by strong fluorescence of the present label and by the extremely suppressed background level brought about by the direct labeling of proteins with the β-diketone-Eu3+ complex. A general consideration and ideas are given for designing ideal label ligands for strongly fluorescent Eu3+ complexes.
A novel biocompatible europium ligand for sensitive time-gated immunodetection
Sayyadi, Nima,Connally, Russell E.,Try, Andrew
supporting information, p. 1154 - 1157 (2016/01/15)
We describe the synthesis of a novel hydrophilic derivative of a tetradentate β-diketone europium ligand that was used to prepare an immunoconjugate probe against Giardia lamblia cysts. We used a Gated Autosynchronous Luminescence Detector (GALD) to obtain high quality delayed luminescence images of cells 30-fold faster than ever previously reported.
Assay of substance in biological sample using labeled probe
-
Referential example 1, (2008/06/13)
A method for analyzing an objective substance, comprsing reacting a labeled probe with an objective substance on a biological sample, said probe comprsing a label substance of the formula (I): wherein A1is an aromatic group, R1is a hydrogen or —COCH2COCnF2n+1and n is an integer of 1-6, which is bonded to a probe selected from the group consisting of nucleic acid, nudeic acid binding potein, low molecular ligand and receptor for ligand (except antibody) to give a fluorescent complex, reacting the complex with an objective substance on a biolgical sample and assaying fluorescence of the resultant fluorescent complex, a labeled nucleic acid probe and a labeled nucleotide. According to the method of the present invention, defects such as hindrance of fluorescence due to contaminant substance, low sensitivity and the like can be resolved, thereby enabling analysis on a tissue.