99103-03-4Relevant articles and documents
Preparation method of chiral bisoprolol fumarate
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Paragraph 0047-0051, (2021/01/20)
The invention provides a preparation method of chiral bisoprolol fumarate. The preparation method comprises the following steps: preparation of (S)-bisoprolol fumarate and preparation of (R)-bisoprolol fumarate. According to the preparation method of the chiral bisoprolol fumarate, provided by the invention, isopropoxyethoxymethylphenol and sulfonyl chloride are subjected to esterification reaction to generate a sulfonate compound, the compound reacts with chiral epoxypropanol to generate a chiral epoxy compound, the compound is subjected to a ring-opening reaction to obtain chiral bisoprolol,and the chiral bisoprolol is subjected to a salt forming reaction to obtain chiral bisoprolol fumarate, wherein the obtained product is high in single configuration purity, so that the method has themain advantages that the raw materials are easy to obtain, the process is simple, economical and environment-friendly, and industrial production is facilitated.
A protein-based mixed selector chiral monolithic stationary phase in capillary electrochromatography
Xu, Shujuan,Wang, Yuying,Tang, Yixia,Ji, Yibing
, p. 13520 - 13528 (2018/08/21)
A new mixed selector chiral stationary phase (CSP) was prepared with co-immobilized human serum albumin and cellulase on a poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EDMA)) monolith and the evaluation of its usefulness in chiral separation research was presented. For comparison, two single selector chiral stationary phases (CSPs) were also fabricated with the corresponding proteins. The enantioseparation ability of these CSPs was investigated by capillary electrochromatography (CEC) with various racemates. The mixed selector CSP exhibited a broader range of enantioselectivities than the single selectors and it could separate 10 chiral analytes while the two single selector CSPs resolved 3 and 8 respectively. Moreover, for (±)-warfarin, the enantioresolution was improved on the mixed selector CSP. Meanwhile, compared with the single selector CSPs, no additional preparation stage or reagent consumption was required in the simultaneous immobilization of different proteins, which is more favorable from economical and practical points of view. Consequently, by mixing HSA and cellulase together, the composite column combines the enantioselectivities of both individual proteins, thus expanding their application range practically.
Chiral separations of some β-adrenergic agonists and antagonists on AmyCoat column by HPLC
Ali, Imran,Saleem, Kishwar,Gaitonde, Vinay D.,Aboul-Enein, Hassan Y.,Hussain, Iqbal
experimental part, p. 24 - 28 (2010/09/14)
Sixteen β-adrenergic antagonists namely acebutalol, alprenolol, atenolol, bisoprolol, bopindolol, bufurolol, carazolol, celiprolol, indenolol, metaprolol, nebivolol, oxprenolol, practolol, propranolol, tertalol, and timolol, and two β-adrenergic agonists namely cimeterol and clenbuterol were resolved on AmyCoat (150 x 46 mm, 3 μm size of silica particle) by using (85:15:0.1, v/v/v), (90:10:0.1, v/v/v), and (95:05:0.1, v/v/v) combinations of η-heptane, ethanol, and diethylamine solvents, respectively. The flow rates used were 0.5, 1.0, 2.0, and 3.0 ml/min with detection at 225 nm. The values of capacity, separation, and resolution factors ranged from 0.38 to 19.70, 1.08-2.33, and 1.0 and 4.50, respectively. The maximum and minimum resolutions were achieved for celiprolol and bufurolol, respectively. The chiral recognition mechanisms were also discussed. The values of validation parameters were calculated.
