99541-89-6

Basic information

• Product Name: Soyasapogenol B 3-O-β-D-glucuronopyranoside
• CAS NO: 99541-89-6
• Molecular Weight: 634.851
• Mol File: 99541-89-6.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: Soyasapogenol B 3-O-β-D-glucuronopyranoside(CAS DataBase Reference)
• NIST Chemistry Reference: Soyasapogenol B 3-O-β-D-glucuronopyranoside(99541-89-6)
• EPA Substance Registry System: Soyasapogenol B 3-O-β-D-glucuronopyranoside(99541-89-6)

Safety Data

• Hazardous Substances Data: 99541-89-6(Hazardous Substances Data)

Upstream product

• 51330-27-9 Soyasaponin I 

Relevant articles and documents

Purification and some properties of soybean saponin hydrolase from Aspergillus oryzae KO-2.

We had investigated the enzymatic hydrolysis of soybean saponins and selected soybean saponin hydrolase from Aspergillus oryzae KO-2. We attempted purification of this enzyme for further characterization. This enzyme was purified 1500-fold using ammonium sulfate fractionation and Sephadex G-200 gel filtrations. The enzyme was electrophoretically homogeneous and a glycoprotein by PAS staining. By gel filtration, the molecular weight of enzyme was 158,000 and SDS-PAGE showed the enzyme to have a tetrameric structure composed of heterogeneous subunits of 35,000 and 45,000. The enzyme activity was stable at temperatures below 40 degrees C and stable from pH 5.0 to 8.0. The optimum pH was pH 4.5 to 5.0 and the optimum temperature was 50 degrees C. The Km and Vmax for soyasaponin I were 0.48 mM and 9.8 mumol/hr mg protein, respectively. After hydrolysis with the enzyme, soyasapogenol B and alpha-L-rhamnopyranosyl (1----2)-beta-D-galactopyranosyl (1----2)-D-glucuronopyranoside were released from soyasaponin I.