506-13-8Relevant articles and documents
Sustainable Method for the Large-Scale Preparation of Fe3O4 Nanocrystals
Lee, SungWoo,Yoon, Jae-Sik,Kang, Sungkyoung,Kwon, Kihyun,Chang, Ki Soo,Lee, SangGap,Choi, Sang-Il,Jeong, Jong-Ryul,Lee, Gaehang,Nam, Ki Min,Davies
, p. 2578 - 2584 (2016)
In this work, a facile synthetic process is reported for the large-scale synthesis of Fe3O4 nanocrystals. Thermal decomposition of Fe(acac)3 (100 g) in 1-hexadecanol produced Fe3O4 nanocrystals with well-controlled sizes and morphologies. The nanocrystals were spherically shaped with average diameters of 7.8 ± 0.6, 6.5 ± 0.4, and 5.9 ± 0.2 nm when prepared at 300°C, 270°C, and 250°C, respectively. Mechanisms of crystal formation were elucidated on the basis of gas chromatography-mass spectroscopy analysis, enabling the large-scale preparation of Fe3O4 nanocrystals. To provide an environmentally benign route, Fe3O4 nanocrystals were prepared with recycled solvent which was recovered from the initial experiment. The resulting porous Fe3O4 nanocrystals had larger average sizes than those of the initial nanocrystals. Structural characterization was performed using transmission electron microscopy and powder X-ray diffraction.
ALKANE OXIDATION BY MODIFIED HYDROXYLASES
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Paragraph 0339, (2016/02/16)
This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.
Dendrimeric alkylated polyethylenimine nano-carriers with acid-cleavable outer cationic shells mediate improved transfection efficiency without increasing toxicity
Steele, Terry W. J.,Shier, Wayne Thomas
experimental part, p. 683 - 698 (2011/03/20)
Purpose. Improved polycation-based non-viral DNA vectors were sought by preparing dendrimers with polyethylenimine cores surrounded by various shells incorporating structural features intended to facilitate steps in transfection mechanisms. Dendrimeric vectors were designed with (a) an outer oligocation shell, intended to facilitate DNA release inside cells, (b) a hydrophobic C-16 alkyl shell, and (c) a polycationic core, the latter two intended to combine lipid-depletion and osmotic burst endosome escape mechanisms, respectively, and were (d) attached through an a acid-cleavable linker reported to hydrolyze at endosomal pH values. Methods. Vectors and DNA complexes were characterized by dynamic and static light scattering. Flow cytometry was used to quantitate transfection activity and cytotoxicity in CHO-Kl cells. Results. About 5-fold increased transfection activity was obtained for a vector constructed with an outer shell of oligocations attached through acid-cleavable linkers, relative to a control dendrimer with an acidstable linker. The most effective oligocation component of outer shells tested was spermine. Neither modification was associated with increased cytotoxicity. This vector design did not permit an evaluation of the benefit of combining endosome release mechanisms. Conclusion. Using acid-cleavable linkers to attach an outer shell of short, highly-charged oligocations to a PEI-based dendrimeric vector substantially increased transfection efficiency without increasing cytotoxicity.