5325-76-8

Basic information

• Product Name: 4,4'-DITHIOBISPHENYLACETIC ACID
• Synonyms: 4,4'-DITHIOBISPHENYLACETIC ACID;4,4'-DITHIOBISBENZENEACETIC ACID, DISULFIDE;(Dithiodi-p-phenylene)di-acetic Acid;NSC 211;NSC 38071
• CAS NO: 5325-76-8
• Molecular Formula: C₁₆H₁₄O₄S₂
• Molecular Weight: 334.41
• Product Categories: Sulfur & Selenium Compounds
• Mol File: 5325-76-8.mol
• Article Data: 6

Chemical Properties

• Boiling Point: 549.4°Cat760mmHg
• Flash Point: 286.1°C
• Appearance: /
• Density: 1.44g/cm³
• Storage Temp.: -20°C Freezer
• Solubility: Acetonitrile (Slightly), DMSO (Slightly), Methanol (Slightly)
• CAS DataBase Reference: 4,4'-DITHIOBISPHENYLACETIC ACID(CAS DataBase Reference)
• NIST Chemistry Reference: 4,4'-DITHIOBISPHENYLACETIC ACID(5325-76-8)
• EPA Substance Registry System: 4,4'-DITHIOBISPHENYLACETIC ACID(5325-76-8)

Safety Data

• Hazardous Substances Data: 5325-76-8(Hazardous Substances Data)

Usage

Description

4,4'-DITHIOBISPHENYLACETIC ACID, also known as DTBP, is an organic compound that serves as a redox buffer. It is characterized by its ability to increase the folding rate of disulfide-containing proteins compared to traditional buffers like glutathione and glutathione-disulfide. This property makes it a valuable tool in various applications across different industries.

Uses

Used in Biochemistry and Molecular Biology:
4,4'-DITHIOBISPHENYLACETIC ACID is used as a redox buffer for increasing the folding rate of disulfide-containing proteins. Its application reason is to enhance protein folding efficiency and improve the overall yield of correctly folded proteins, which is crucial for various research and diagnostic applications.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, 4,4'-DITHIOBISPHENYLACETIC ACID is used as a stabilizing agent for protein-based drugs. The application reason is to improve the stability and shelf life of these drugs by maintaining the correct disulfide bond formation and preventing protein aggregation.
Used in Diagnostics:
4,4'-DITHIOBISPHENYLACETIC ACID is used as a component in diagnostic assays that require the detection or quantification of disulfide-containing proteins. The application reason is to ensure accurate and reliable results by providing an optimal redox environment for protein folding and stability.
Used in Research and Development:
In research and development, 4,4'-DITHIOBISPHENYLACETIC ACID is used as a tool for studying protein folding and disulfide bond formation. The application reason is to gain a better understanding of the underlying mechanisms and to develop new strategies for protein engineering and drug design.
Overall, 4,4'-DITHIOBISPHENYLACETIC ACID is a versatile compound with a wide range of applications in various industries, primarily due to its ability to act as a redox buffer and improve the folding rate of disulfide-containing proteins.

Upstream product

• 39161-84-7 4-mercaptophenylacetic acid 
• 95-71-6 2-methylbenzene-1,4-diol 
• 824-46-4 methoxyhydroquinone 

Relevant articles and documents

Folding disulfide-containing proteins faster with an aromatic thiol

The traditional method for in vitro folding of disulfide-containing proteins is slow and involves a redox buffer of glutathione and glutathione disulfide. To increase the folding rate and to gain insight into the folding process, we replaced glutathione, an aliphatic thiol, with a commercially available aromatic thiol, 4-mercaptobenzeneacetate (1). Aromatic thiol 1 was selected due to its enhanced nucleophilicity and its enhanced leaving-group ability relative to glutathione at pH 7.7. To demonstrate the advantages of 1, the folding of reduced and scrambled RNase A at pH 7.0 and 7.7 in the presence of 1 and glutathione was investigated. For each set of folding conditions, the optimum concentration of each thiol was initially determined and then the folding rates in the presence of each thiol were measured concurrently. In all cases examined, the folding rate enhancement with the aromatic thiol was 5- 6-fold. Furthermore, under similar conditions folding rates were almost identical with either reduced or scrambled RNase A. In addition the 5-6-fold folding rate enhancement varied only slightly with pH, 7.0 vs 7.7.

METHOD FOR PATHOGENS, MICROORGANISMS, AND PARASITES INACTIVATION

The invention provides a method for inactivation or reduction of pathogens, microorganisms or parasites in a sample, media, composition, utility, device, surface or organism by treatment with an alkylating compound of Structure I, followed by elimination or reduction of the residual compound with Structure I by treatment with a neutralizing agent, which eliminates or reduces the toxicity or other undesirable properties of the alkylating compound with Structure I. The neutralizing agent may be present in a treatment solution or be part of a solid-phase agent, and preferably acts by eliminating the alkylating properties of the compound of Structure I.

Enzymatic thiol Michael addition using laccases: Multiple C-S bond formation between p-hydroquinones and aromatic thiols

Laccases create C-C, C-O or C-N bonds and have been investigated intensively as catalysts for green chemistry and white biotechnology. However, little is known about C-S bond formation in laccase-catalyzed reactions. We have used the laccases from Pycnopo