609-99-4 Usage
Description
3,5-Dinitrosalicylic acid is a derivative of salicylic acid, characterized by the presence of nitro substituents at the 3and 5-positions on the 2-hydroxybenzoic acid structure. It is a solid compound that is utilized in various colorimetric tests and applications, particularly for the detection and quantification of reducing sugars and free carbonyl groups.
Uses
Used in Analytical Chemistry:
3,5-Dinitrosalicylic acid is used as a colorimetric reagent for the determination of reducing sugars. It is particularly useful in the quantification of reducing sugars by colorimetry, which is essential for the analysis of glycosidase (glycoside hydrolase) activity by measuring the enzymatically released reducing sugar.
Used in Enzyme Activity Analysis:
In the field of biochemistry, 3,5-dinitrosalicylic acid is employed to analyze the activity of glycosidases. By quantifying the enzymatically released reducing sugar, this compound helps researchers understand the function and efficiency of these enzymes in various biological processes.
Used in Medical Diagnostics:
3,5-Dinitrosalicylic acid is also used to detect reducing substances in urine and for quantifying carbohydrate levels in blood. These applications are crucial for diagnosing and monitoring various medical conditions related to carbohydrate metabolism and renal function.
Used in Pharmaceutical and Agrochemical Production:
3,5-Dinitrosalicylic acid serves as an important raw material and intermediate in the production of organic chemicals, including those used in the pharmaceutical, agrochemical, organic synthesis, and dye industries. Its versatility in these applications stems from its ability to react with reducing sugars and other carbonyl-containing compounds.
Used in Spectrophotometric Determinations:
In addition to its use in colorimetric tests, 3,5-dinitrosalicylic acid has been utilized as a reagent for the preparation of oxazolines from amino alcohols and for the spectrophotometric determination of ampicillin. It has also been employed to measure the effects of silver nanoparticles on the membrane leakage of reducing sugars, demonstrating its utility in various research and analytical applications.
Biochem/physiol Actions
3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.
Purification Methods
Crystallise the acid from H2O. [Beilstein 10 IV 270.]
Check Digit Verification of cas no
The CAS Registry Mumber 609-99-4 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 6,0 and 9 respectively; the second part has 2 digits, 9 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 609-99:
(5*6)+(4*0)+(3*9)+(2*9)+(1*9)=84
84 % 10 = 4
So 609-99-4 is a valid CAS Registry Number.
InChI:InChI=1/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)/p-2
609-99-4Relevant articles and documents
Intramolecular base catalysed hydrolysis of ortho-hydroxyaryl esters: The anomalous position of methyl 3,5-dinitrosalicylate on the Linear Free Energy Relationship plot
Moozyckine, Alexei U.,Davies, D. Martin
, p. 1158 - 1161 (2002)
The pKa of the ortho-hydroxy group in methyl 3,5-dinitrosalicylate, HMDNS, is 2.45. Rate constants for the reaction of its conjugate base, MDNS- with hydroxide anion and water are 5.3 × 10-2 mol dm-3 s-1 and 6.6 × 10-6 s-1, respectively at 25°C. The rate constant for the uncatalysed reaction of HMDNS and water is 6.5 × 10-6 s-1 and so there is no evidence for intramolecular general base catalysis of the water reaction with MDNS- by the weakly basic ortho-O-. By means of Bronsted plots the water reaction of MDNS- is compared with that of a group of other salicylate esters (β = 0) and also a structurally different group of esters (β = 0.4), both of which undergo intramolecular base catalysed hydrolysis. Although the title ester structurally belongs to the first set of compounds, its anomalous position on the plot clearly corresponds to the trend of the second set. This is explained in terms of differences in resonance stabilisation and hydrogen bonding in the transition state.
NOVEL WATER SOLUBLE POLYIMIDE RESIN, ITS PREPARATION AND USE
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, (2011/08/03)
The present invention relates to a novel water soluble polyimide resin, which contains a hydrophilic functional group such as —OH, —COOH to increase the solubility of the polyimide resin in alkali aqueous solution, and is suitable for using as an insulation film in electronic and photoelectric products. The present invention also relates to preparation and use of the above polyimide.
Process for the synthesis of hydroxy aromatic acids
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Page/Page column 20-21, (2008/06/13)
Hydroxy aromatic acids are produced in high yields and high purity (>95%) from halogenated aromatic acids in a reaction mixture containing a copper source and a ligand that coordinates to copper.
Nitration of aromatic compounds by Zn(NO3)2· 2N2O4 and its charcoal-supported system
Iranpoor, Nasser,Firouzabadi, Habib,Heydari, Reza,Shiri, Morteza
, p. 263 - 270 (2007/10/03)
Zn(NO3)2·N2O4 and its charcoal supported system were found to be efficient nitrating agents. Mononitration of aromatic compounds such as benzene, alkyl benzenes, halobenzenes, nitrobenzene, anisol, and the highly selective mono-, di-, and trinitration of phenol, and dinitraion of substituted phenols were also performed in the presence of these reagents.