63760-59-8Relevant articles and documents
Isolation and Properties of 7β-(4-Carboxybutanamido)cephalosporanic Acid Acylase-producing Bacteria
Shibuya, Yuzo,Matsumoto, Kunio,Fuji, Tadashiro
, p. 1561 - 1568 (2007/10/02)
A search was undertaken for microorganisms that produce an enzyme capable of deacylating 7β-(4-carboxybutanamido)cephalosporanic acid (glutaryl 7-ACA, GL-7-ACA) to 7-aminocephalosporanic acid.Pseudomonas putida ATCC 950 was selected from our stock cultures, and Pseudomonas SY-77-1 was isolated from soil.Adding glutaric acid to the growth media enhanced the production of GL-7-ACA acylase by Pseudomonas SY-77-1.A β-lactamase-deficient mutant, Pseudomonas NS-2, was derived from Pseudomonas SY-77-1 treated with a mutagen.The cell suspension of Pseudomonas NS-2 specifically hydrolyzed cephalosporin compounds having aliphatic dicarboxylic acid in acyl side chain.