135795-62-9

Basic information

• Product Name: NHS-FLUORESCEIN
• Synonyms: NHS-FLUORESCEIN
• CAS NO: 135795-62-9
• Molecular Formula: C₂₄H₁₅NO₇
• Molecular Weight: 429.38
• Mol File: 135795-62-9.mol
• Article Data: 4

Chemical Properties

• Boiling Point: 720.6±70.0 °C(Predicted)
• Appearance: /
• Density: 1.58±0.1 g/cm3(Predicted)
• PKA: 9.05±0.20(Predicted)
• CAS DataBase Reference: NHS-FLUORESCEIN(CAS DataBase Reference)
• NIST Chemistry Reference: NHS-FLUORESCEIN(135795-62-9)
• EPA Substance Registry System: NHS-FLUORESCEIN(135795-62-9)

Safety Data

• Hazardous Substances Data: 135795-62-9(Hazardous Substances Data)

Usage

General Description

NHS-Fluorescein is a chemical compound commonly used in biological and medical research as a fluorescent labeling reagent. It is a derivative of fluorescein, a fluorescent dye, and is often used to label and track molecules in biological systems. NHS-Fluorescein is reactive towards amine groups, allowing it to easily bind to proteins and other biomolecules, making it a valuable tool for studying biological processes. Its fluorescent properties enable researchers to visualize and track the movement and localization of labeled molecules, providing valuable insights into the dynamics of biological systems.

Upstream product

• 6066-82-6 1-hydroxy-pyrrolidine-2,5-dione 
• 518-45-6 fluorescein free acid 
• 2321-07-5 fluorescein 

Downstream Products

• 442151-46-4 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-N,N-dimethylbenzamide 
• 442151-48-6 [9-(2-dimethylcarbamoylphenyl)-6-oxo-6H-xanthen-3-yloxy]acetic acid methyl ester 
• 1355342-72-1 C₂₉H₃₀N₂O₆ 
• 1446245-17-5 4-(4-(2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoyl)piperazin-1-yl)-4-oxo-N-phenylbutanamide 
• 1415730-00-5 N-[4-(1,3,2-dithiarsolan-2-yl)phenyl]-4-{4-[2-(6-hydroxy-3-oxo-3H-xan-then-9-yl)benzoyl]piperazin-1-yl}-4-oxobutanamide 

Relevant articles and documents

An in-situ detection of biological in vivo adjacent mercat protein compound and its method of

The invention belongs to the technical field of biological detection, and specifically relates to a compound and a method for in-situ detecting o-sulfhydryl protein in an organism. A kind of micromolecular fluorescent probes capable of specifically detecting o-sulfhydryl of protein are designed and synthesized, and a method for in-situ fluorescence labeling and detection of endogenous o-sulfhydryl protein in the organism is built by combining fluorescence polarization technology, protein fluorescent electrophoresis and confocal fluorescence microtechnique.

A simple FRET-based modular design for diagnostic probes

In recent years, there has been a massive effort to develop molecular probes with optical modes of action. Probes generally produce detectable signals based on changes in fluorescence properties. Here, we demonstrate the potential of self-immolative molecular adaptors as a platform for Turn-On probes based on the FRET technique. The probe is equipped with identical fluorophore pairs or a fluorophore/quencher FRET pair and a triggering substrate. Upon reaction of the analyte of interest with the triggering substrate, the self-immolative adaptor spontaneously releases the two dye molecules to break off the FRET effect. As a result, a new measurable fluorescent signal is generated. The fluorescence obtained can be used to quantify the analyte. The modular structure of the probe design will allow the preparation of various chemical probes based on the FRET activation technique.