145-12-0 Usage
Chemical Properties
White Solid
Originator
Anamidol,Iwaki
Uses
An anabolic steroid.
Manufacturing Process
2 Methods of producing of 4-hydroxy-17α-methyltestosterone:
1. A solution of 1.0 g of crude 4,5-oxido-17α-methyltestosterone in 50 ml of methanol is allowed to stand at room temperature overnight with 10 ml of water and 1 ml of concentrated sulfuric acid. It is then poured into water containing sodium chloride and extracted three times with ethyl acetate. The solvent is washed with water, then with 10% sodium bicarbonate solution and again with water to neutrality. The residue remaining after evaporation of the solvent is crystallized from methanol, giving 17α-methyl-androstane4β,5α,17β-triol-3-one with a melting point of 203°-205°C.
A solution of 0.22 g of 17α-methyl-androstane-4β,5α,17β-triol-3-one in 100 ml of methanol is allowed to stand at room temperature for 22 h, under nitrogen, with 0.30 g of potassium hydroxide in 4 ml of water and 20 ml of methanol. The solution is then neutralized with acetic acid, concentrated in vacuo, diluted with water and extracted three times with ethyl acetate. The extract is washed with water and the solvent removed by distillation. The remaining residue is chromatographed over Florisil 30-60 mesh. The fractions eluted with benzene and benzene-ether (10:1) are combined and by crystallization from ether-petroleum ether give 4-hydroxy-17α-methyltestosterone (0.120 g) melting at 168°-170°C.
2. A solution of 20.0 g of 17α-testosterone in 500 ml of trimethylcarbinol is treated by addition of 56 ml of 30% hydrogen peroxide and 1.0 g of osmium tetroxide in 80 ml of trimethylcarbinol. After the mixture has stood at room temperature for 22 h, 12 ml of hydrogen peroxide are added. The reaction mixture is allowed to stand at room temperature for an additional 20 h, then concentrated in vacuo to 1/3 of its original volume, diluted with water, and the reaction product extracted with ethyl acetate. The extract is washed with water, several times with 10% sodium bisulfite solution, then with 4% sodium bicarbonate solution and finally with water to neutrality. The residue remaining after evaporation of the solvent does not show ultraviolet absorption. 1.0 g of this crude substance, by crystallization from methanol, gives l7αmethylandrostane-4,5,17β-triol-3-one (0.400 g) melting at 192°-194°C.
A solution of 20.0 g of crude 17β-methylandrostane-4,5,17β-triol-3-one in 1 L of methanol is heated under reflux in a stream of nitrogen for 20 min; then 20.0 g of potassium hydroxide in 40 ml of water and 200 ml of methanol are added. 5 min after the addition, the solution is treated by addition of 20 ml of acetic acid and concentrated in vacuo. The residue is diluted with water containing sodium chloride and extracted three times with ethyl acetate. The extract is washed with 10% sodium bicarbonate solution and then with water to neutrality. The residue remaining after evaporation of the solvent is
dissolved in acetone; addition of petroleum ether gives 4-hydroxy-17αmethyl-testosterone (8.0 g) melting at 168°-170°C. The mother liquors chromatographed over Florisil 30-60 mesh yield an additional 5.0 g of the same substance melting at 168°-170°C.
Therapeutic Function
Anabolic, Androgen
Check Digit Verification of cas no
The CAS Registry Mumber 145-12-0 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 1,4 and 5 respectively; the second part has 2 digits, 1 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 145-12:
(5*1)+(4*4)+(3*5)+(2*1)+(1*2)=40
40 % 10 = 0
So 145-12-0 is a valid CAS Registry Number.
InChI:InChI=1/C20H30O3/c1-18-9-8-16(21)17(22)15(18)5-4-12-13(18)6-10-19(2)14(12)7-11-20(19,3)23/h12-14,22-23H,4-11H2,1-3H3/t12-,13+,14+,18-,19+,20+/m1/s1
