150-25-4 Usage
Description
Bicine, also known as N,N-bis(2-hydroxyethyl)glycine, is a zwitterionic amino acid buffer agent that is widely used in various applications due to its unique properties. It is a white/clear crystalline powder that offers versatility in different industries.
Uses
Used in Enzyme Assays:
Bicine is used as a stable substrate solution for the determination of guanine deaminase, providing a reliable and consistent environment for enzyme activity measurements.
Used in Biological Research:
As a biological buffer and chelating agent, Bicine is employed to maintain the stability of biological samples and prevent interference from metal ions during experiments.
Used in Low Temperature Work:
Bicine is used as a buffer agent for low temperature work, ensuring the stability and functionality of biological molecules at lower temperatures.
Used in Chromatography:
In the field of chromatography, Bicine is used as a buffer agent in thin layer ion exchange chromatography methods for protein resolution, enhancing the separation and identification of proteins.
Used in Protein Crystallization:
Bicine is utilized in peptide and protein crystallization processes, facilitating the formation of high-quality crystals for structural analysis.
Used in Kinetic Studies:
In a kinetic study of a quaternary transition-state analogue complex of creatine kinase, Bicine was used in the reaction buffer, highlighting its importance in understanding enzyme mechanisms.
Used in SDS-PAGE:
Bicine is a component of a multiphasic buffer system for SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) of proteins and peptides, improving the separation and analysis of these biomolecules.
Purification Methods
Dissolve bicine in a small volume of hot water and precipitate it with EtOH, twice. Repeat once more but treat the aqueous solution with charcoal Purification of Biochemicals — Amino Acids and Peptides and filter before adding EtOH. Also crystallise it from concentrated aqueous solutions. [Torn & Kolthoff J Am Chem Soc 77 2061 1955, Chaberek et al. J Am Chem Soc 75 2185 1953, Beilstein 4 IV 2390.]
Check Digit Verification of cas no
The CAS Registry Mumber 150-25-4 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 1,5 and 0 respectively; the second part has 2 digits, 2 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 150-25:
(5*1)+(4*5)+(3*0)+(2*2)+(1*5)=34
34 % 10 = 4
So 150-25-4 is a valid CAS Registry Number.
InChI:InChI=1/C2H5NO2.2C2H6O/c3-1-2(4)5;2*1-2-3/h1,3H2,(H,4,5);2*3H,2H2,1H3
150-25-4Relevant articles and documents
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Hardegger,Ott
, p. 213 (1955)
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Isolation of nucleic acids
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, (2008/06/13)
A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing.
Facile hydrolysis and formation of amide bonds by N-hydroxyethylation of α-amino acids
Suggs, J. William,Pires, Richard M.
, p. 2227 - 2230 (2007/10/03)
The C-terminal amides of α-amino acids are readily hydrolyzed at 25°and pH 7 when the N-terminus is N-hydroxyethylated, with one or two hydroxyethyl The reaction proceeds via cyclization to a morpholinolactone (2) which is rapidly hydrolyzed by water. In the presence of equimolar amounts of amines or amino acid derivatives, 2 reacts in H2O without condensing agents to form a new peptide bond.
