21640-24-4Relevant articles and documents
Regulating miRNA-21 Biogenesis by Bifunctional Small Molecules
Yan, Hao,Bhattarai, Umesh,Guo, Zhi-Fo,Liang, Fu-Sen
, p. 4987 - 4990 (2017)
We report a new strategy to regulate microRNAs (miRNAs) biogenesis by using bifunctional small molecules that consist of a pre-miRNA binding unit connected by a linker to a Dicer inhibiting unit. In this effort, fluorescence polarization-based screening was used to identify neomycin as a pre-miR-21 binding ligand. Although neomycin cannot inhibit miR-21 maturation, linking it to the RNase inhibitor 1 forms the bifunctional conjugate 7A, which inhibits the production of miR-21. We expect that this strategy will be applicable to design other molecules for miRNA regulation.