220141-70-8

Basic information

• Product Name: 5-FLUOROOROTIC ACID HYDRATE 98
• Synonyms: 5-FLUOROOROTIC ACID HYDRATE 98;5-Fluoro-2,6-dioxo-1,2,3,6-tetrahydropyriMidine-4-carboxylic acid hydrate;5-FOA (5-Fluoroorotic acid), Monohydrate
• CAS NO: 220141-70-8
• Molecular Formula: C₅H₃FN₂O₄*H₂O
• Molecular Weight: 192.1020032
• EINECS: 211-876-0
• Mol File: 220141-70-8.mol
• Article Data: 1

Chemical Properties

• Melting Point: 278 °C (dec.)(lit.)
• Boiling Point: °Cat760mmHg
• Flash Point: °C
• Appearance: /
• Density: 1.81g/cm³
• Storage Temp.: Inert atmosphere,Store in freezer, under -20°C
• Solubility: Aqueous Base (Heated), DMSO (Sparingly, Heated), Methanol (Sparingly), Water (Sl
• Water Solubility: Soluble in water, dimethyl sulfoxide, 50mg in 1ml (1:1 ammonium hydroxide: water) with gentle heating.
• Merck: 14,4176
• BRN: 181933
• CAS DataBase Reference: 5-FLUOROOROTIC ACID HYDRATE 98(CAS DataBase Reference)
• NIST Chemistry Reference: 5-FLUOROOROTIC ACID HYDRATE 98(220141-70-8)
• EPA Substance Registry System: 5-FLUOROOROTIC ACID HYDRATE 98(220141-70-8)

Safety Data

• Hazard Codes: Xn
• Statements: 22-36/37/38
• Safety Statements: 26-36
• WGK Germany: 3
• RTECS: UV7800000
• HazardClass: IRRITANT
• Hazardous Substances Data: 220141-70-8(Hazardous Substances Data)

Usage

Description

5-Fluoroorotic Acid Hydrate 98, also known as 5-FOA, is a chemical compound that has gained significant importance in the field of molecular genetics. It is primarily used as a tool for detecting URA3 gene expression in yeast molecular genetic constructs. The compound is particularly effective in identifying yeast strains with a mutant URA3 gene, which renders them resistant to the toxic effects of 5-FOA.

Uses

1. Used in Molecular Genetics Research:
5-Fluoroorotic Acid Hydrate 98 is used as a research tool for detecting URA3 gene expression in yeast molecular genetic constructs. It helps identify yeast strains with a mutant URA3 gene, which is crucial for understanding the genetic mechanisms and potential applications in various fields.
2. Used in Selection of Resistant Yeast Strains:
5-Fluoroorotic Acid Hydrate 98 is employed in the selection of resistant strains of Saccharomyces cerevisiae that possess a mutant URA3 gene. This gene mutation allows the yeast to grow in the presence of 5-FOA, making it an essential component in the development of genetically modified yeast strains for various applications.
3. Used in Yeast Media Supplementation:
5-Fluoroorotic Acid Hydrate 98 is used as a supplement in yeast media to study the effects of URA3 gene expression on yeast growth and survival. By supplementing the media with uracil, researchers can observe the growth of yeast strains with a mutant URA3 gene in the presence of 5-FOA, providing valuable insights into the genetic and molecular mechanisms involved.

Upstream product

• 50887-69-9 2,6-dioxo-1,2,3,6-tetrahydropyrimidine-4-carboxylic acid monohydrate 

Relevant articles and documents

Process for producing 5-fluorouracil

5-Fluorouraacil is produced by reacting orotic acid with a fluorinating agent and then, decarboxylating the resulting 5-fluoro-orotic acid.