298-96-4 Usage
Description
2,3,5-Triphenyltetrazolium chloride, also known as TTC, is a colorless water-soluble dye that is widely used in various fields due to its unique properties. It is a white powder that, in the mitochondria of living cells, is reduced to a deep red, water-insoluble compound called formazan. This characteristic helps to distinguish between viable and infarcted brain tissue after stroke.
Uses
Used in Cell Biological Studies:
2,3,5-Triphenyltetrazolium chloride is used as a dye in cell biological studies for its ability to indicate cell viability and assess the metabolic activity of cells. It is particularly useful in distinguishing between living and dead cells, making it a valuable tool in research and development.
Used in Pharmaceutical Industry:
2,3,5-Triphenyltetrazolium chloride is used as a selective serotonin 5-HT4 receptor agonist and 5-HT3 gastrointestinal prokinetic agent for functional dyspepsia. This application aids in the treatment of gastrointestinal disorders by targeting specific receptors and improving motility.
Used in Analytical Chemistry:
In analytical chemistry, TTC is employed as a sensitive reagent for detecting reducing sugars and differentiating between α-keto acids and simple aldehydes. Its ability to produce a color change upon interaction with these compounds makes it an essential tool in various analytical techniques.
Used in Staining Plant and Animal Tissue:
2,3,5-Triphenyltetrazolium chloride is utilized for staining plant and animal tissues, allowing for the visualization of cellular structures and facilitating the study of tissue morphology.
Used as a Germination Indicator in Seed Testing:
TTC serves as a germination indicator in testing the ability of seeds to germinate. This application is crucial in agriculture and horticulture for assessing seed viability and ensuring successful plant growth.
Used in Histochemical Studies:
In histochemical studies, 2,3,5-Triphenyltetrazolium chloride is used to identify and analyze various cellular components and processes, contributing to a better understanding of cell function and structure.
Used in Determination of Antibiotics and Dehydrogenases:
TTC is also used in the determination of antibiotics and dehydrogenases, playing a vital role in the development and assessment of these essential compounds in the medical and pharmaceutical fields.
Purification Methods
Crystallise TTZ from EtOH or CHCl3, and dry it at 105o. [Beilstein 26 H 363, 26 II 216, 26 III/IV 1774.]
Check Digit Verification of cas no
The CAS Registry Mumber 298-96-4 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 2,9 and 8 respectively; the second part has 2 digits, 9 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 298-96:
(5*2)+(4*9)+(3*8)+(2*9)+(1*6)=94
94 % 10 = 4
So 298-96-4 is a valid CAS Registry Number.
298-96-4Relevant articles and documents
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Mattson,Jensen,Dutcher
, p. 1284 (1948)
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Method for preparing chlorinated-3-substituted-2,5-diphenyl tetrazole under catalysis of copper Lewis acid surfactant
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Paragraph 0057; 0058; 0059; 0064; 0065, (2018/09/12)
The invention belongs to the technical field of organic synthesis and specifically relates to a method for preparing chlorinated-3-substituted-2,5-diphenyl tetrazole under the catalysis of a copper Lewis acid surfactant. The method comprises the following steps: 1) reacting benzaldehyde, phenylhydrazine, arylamine and sodium nitrite in solvent water by taking a copper Lewis acid surfactant Cu(OSO2CnH2n+1)2 as a catalyst to obtain 3-aryl-2,5-diphenylformazan; and 2) cyclizing and chlorinating 3-aryl-2,5-diphenylformazan in a mixed solvent of dichloromethane and water under the condition of a solid chlorination reagent N-chlorosuccinimide to obtain chlorinated-3-substituted-2,5-diphenyl tetrazole. By using the method, three-step conversion is completed according to a ''one-pot method'' continuous strategy, not only is an intermediate separation step omitted, but also the purification step is simple as well as convenient and easy to operate.
Imidazole compounds
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, (2008/06/13)
A novel class of imidazo heterocyclic compounds, pharmaceutical compositions comprising them and use thereof in the treatment and/or prevention of diseases and disorders related to the histamine H3 receptor. More particularly, the compounds are useful for the treatment and/or prevention of diseases and disorders in which an interaction with the histamine H3 receptor is beneficial.
1-Chlorobenzotriazole-Mediated Ring Closure of 1,3,5-Triarylformazans: Improved Syntheses of 2,3,5-Triaryl-2H-tetrazolium Salts
Katritzky, Alan R.,Belyakov, Sergey A.,Lam, Jamshed N.,Durst, H. Dupont,Karpenko, Dmitrii V.
, p. 73 - 80 (2007/10/02)
Oxidative ring closure of mono- and bis-triarylformazans mediated by easily available 1-chlorobenzotriazole leads to the corresponding 2,3,5-triaryl-2H-tetrazolium chlorides in yields of 70-97percent.
