333-31-3 Usage
Description
Methacholine bromide, also known as acetyl-β-methylcholine bromide or provocholine, is a muscarinic acetylcholine receptor agonist. It is a synthetic compound that mimics the action of acetylcholine, a neurotransmitter that plays a key role in various physiological processes. Methacholine bromide is known for its ability to induce bronchoconstriction, making it a valuable tool in medical diagnostics.
Uses
Used in Medical Diagnostics:
Methacholine bromide is used as a diagnostic tool for bronchial hyperreactivity. It helps in identifying and assessing the severity of conditions such as asthma and chronic obstructive pulmonary disease (COPD) by inducing bronchoconstriction and measuring the patient's response.
Used in Pharmaceutical Research:
Methacholine bromide is used as a reference material in studies involving the identification, characterization, and mechanism of action of muscarinic acetylcholine receptors. This helps researchers understand the role of these receptors in various physiological processes and develop potential therapeutic agents targeting them.
Used in Quality Control and Analytical Procedures:
Methacholine bromide may be used as a reference material in procedures designed to detect or quantify its presence. This is important for ensuring the accuracy and reliability of test results in research and quality control applications.
Mechanism of action
A minor structural change is the presence of a methyl group at the β-carbon atom of
choline, which results in two main changes in the pharmacological profile of the molecule.
Unlike acetylcholine, methacholine is hydrolyzed only by acetylcholinesterase, and the
rate of hydrolysis is significantly less than with acetylcholine. Thus, the action of methacholine is significantly longer lasting than acetylcholine. Moreover, the presence of a
methyl group at the β-carbon of choline provides the compound with a greater selectivity
of action. Methacholine directly acts on muscarinic receptors of smooth muscle, glands,and the heart, and it has a very weak effect on nicotinic receptors of the autonomic ganglions of skeletal muscle.
Synthesis
Methacholine, 1-acetoxy-2-(N,N,N-trimethyl)propyl ammonium chloride
(13.1.4) or acetyl-β-methylcholine can be synthesized by any of the methods described
above [8]
Check Digit Verification of cas no
The CAS Registry Mumber 333-31-3 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 3,3 and 3 respectively; the second part has 2 digits, 3 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 333-31:
(5*3)+(4*3)+(3*3)+(2*3)+(1*1)=43
43 % 10 = 3
So 333-31-3 is a valid CAS Registry Number.
InChI:InChI=1/C8H18NO2/c1-7(11-8(2)10)6-9(3,4)5/h7H,6H2,1-5H3/q+1/t7-/m1/s1
