34487-61-1

Basic information

• Product Name: Phenol Red sodium salt
• Synonyms: 3,3-BIS(P-HYDROXYPHENYL)-2,1-3H-BENZOXATHIOLE SODIUM SALT;SODIUM PHENOL RED;PHENOLSULFONEPHTHALEIN MONOSODIUM SALT;PHENOLSULFONEPHTHALEIN SODIUM SALT;PHENOLSULFONPHTHALEIN NA-SALT;PHENOLSULFONPHTHALEIN, SODIUM SALT;PHENOLSULFONPHTHALEIN;PHENOLSULPHONEPHTHALEIN SODIUM SALT
• CAS NO: 34487-61-1
• Molecular Formula: C₁₉H₁₃O₅S*Na
• Molecular Weight: 376.36
• EINECS: 252-057-8
• Product Categories: Analytical Chemistry;Indicator (pH);pH Indicators;Sulfonephthalein
• Mol File: 34487-61-1.mol
• Article Data: 0

Chemical Properties

• Melting Point: 285 °C (dec.)(lit.)
• Boiling Point: 562.8oC at 760mmHg
• Flash Point: 294.2oC
• Appearance: White/Shiny Powder
• Density: 0.972
• Storage Temp.: Store at RT.
• Solubility: H2O: clarity passes test
• PKA: 7.69, 7.9, 1.3(at 25℃)
• Water Solubility: soluble
• Stability: Stable. Incompatible with strong oxidizing agents.
• Merck: 14,7247
• BRN: 6260026
• CAS DataBase Reference: Phenol Red sodium salt(CAS DataBase Reference)
• NIST Chemistry Reference: Phenol Red sodium salt(34487-61-1)
• EPA Substance Registry System: Phenol Red sodium salt(34487-61-1)

Safety Data

• Hazard Codes: Xi,C
• Statements: 36/37/38-34
• Safety Statements: 26-36-37/39-45-36/37/39-27
• WGK Germany: 3
• TSCA: Yes
• Hazardous Substances Data: 34487-61-1(Hazardous Substances Data)

Usage

Chemical Properties

red liquid or red/brown crystals, slightly soluble in water, alcohol and acetone. Almost insoluble in ether and chloroform, soluble in hydroxide base or carbonate base solution with deep red color, stable in air, used as acid-base indicator.

Uses

Different sources of media describe the Uses of 34487-61-1 differently. You can refer to the following data:
1. Phenol Red Sodium Salt is a water soluble pH indicator commonly employed in cell biology laboratories as a cell tissue culture pH marker. Phenol Red sodium salt is a water soluble pH indicator used in the 6.8 (yellow) to 8.2 (red) range. It is commonly employed in cell biology laboratories as a cell tissue culture pH marker. Under normal conditions, phenol red added to the growth medium will have a pink-red color. Usually 15 mg/l is used to produce this effect. If problems arise, such as dying cells or overgrowth of contaminants, this will cause a change in pH resulting in a change in indicator color. This color change is an indication that the medium needs to be replaced. Additionally, Phenol Red could potentially be useful as a differentiation factor. It can also be used as reactant/reag
2. Phenol Red sodium salt has been used as an indicator during single-guide RNA microinjection into one-cell stage zebrafish embryos.
3. Phenol Red has been used for preparation of phosphate buffer saline (PBS)-with phenol red for microfluidic assay for simultaneous culture of multiple cell types. It has also been used for horseradish peroxidase-phenol red (HRP-PR) H2O2 (hydrogen peroxide) detection assay to identify redox cycling compounds.

General Description

Phenol Red sodium salt is widely used as a pH indicator. At a pH of 6.4 and below, phenol red solution will have a yellow color. At a pH of 8.2 and above, it will have a red color. It is commonly used in culture media to detect neutral to acidic pH changes.

InChI:InChI=1/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24/h1-12,20H,(H,22,23,24)/p-1

Synthetic route

phenolsulphophthaleine sodium salt (34487-61-1) → 2-(4,4'-dihydroxy-benzhydryl)-benzenesulfonic acid (172464-36-7)

Conditions	Yield
With hydrogenchloride; sodium tetrahydroborate In ethanol at 25℃; for 48h;

phenolsulphophthaleine sodium salt (34487-61-1) → Bis(phenol) phenyl sulfonate (115481-73-7)

Conditions	Yield
Multi-step reaction with 5 steps 1: 1.) NaBH4, 2.) 5percent HCl / aq. ethanol / 48 h / 25 °C 2: 72 percent / 1.) RT, 4h, 2.) CH2Cl2, 1 h 3: PCl5 / 3 h / 60 - 65 °C / 0.1 Torr 4: 92 percent / Et3N / CH2Cl2 / 12 h / 25 °C 5: 93 percent / HCl conc. / methanol / 20 h / Ambient temperature View Scheme

phenolsulphophthaleine sodium salt (34487-61-1) → bis(4.acetoxyphenyl)<2-(phenoxysulfonyl)phenyl>methane (115511-92-7)

Conditions	Yield
Multi-step reaction with 4 steps 1: 1.) NaBH4, 2.) 5percent HCl / aq. ethanol / 48 h / 25 °C 2: 72 percent / 1.) RT, 4h, 2.) CH2Cl2, 1 h 3: PCl5 / 3 h / 60 - 65 °C / 0.1 Torr 4: 92 percent / Et3N / CH2Cl2 / 12 h / 25 °C View Scheme

phenolsulphophthaleine sodium salt (34487-61-1) → Acetic acid 4-[(4-acetoxy-phenyl)-(2-chlorosulfonyl-phenyl)-methyl]-phenyl ester (147510-56-3)

Conditions	Yield
Multi-step reaction with 3 steps 1: 1.) NaBH4, 2.) 5percent HCl / aq. ethanol / 48 h / 25 °C 2: 72 percent / 1.) RT, 4h, 2.) CH2Cl2, 1 h 3: PCl5 / 3 h / 60 - 65 °C / 0.1 Torr View Scheme

phenolsulphophthaleine sodium salt (34487-61-1) → pyridinium 2-benzenesulfonate (115481-75-9)

Conditions	Yield
Multi-step reaction with 2 steps 1: 1.) NaBH4, 2.) 5percent HCl / aq. ethanol / 48 h / 25 °C 2: 72 percent / 1.) RT, 4h, 2.) CH2Cl2, 1 h View Scheme

2-[2-[4-(dimethylamino)phenyl]ethenyl]-1-ethylquinolinium chloride (65201-92-5) + phenolsulphophthaleine sodium salt (34487-61-1) → QR+*PR-

Conditions	Yield
In water

quinaldine blue (2768-90-3) + phenolsulphophthaleine sodium salt (34487-61-1) → PNC+*PR-

Conditions	Yield
In water

phenolsulphophthaleine sodium salt (34487-61-1) → 3',3'',5',5''-tetrabromophenolsulphonephthalein sodium salt (62625-28-9)

Conditions	Yield
With Agrocybe aegerita heme-thiolate peroxygenase; dihydrogen peroxide; sodium bromide at 20℃; pH=5; Reagent/catalyst; Enzymatic reaction;

Downstream Products

• 62625-28-9 3',3'',5',5''-tetrabromophenolsulphonephthalein sodium salt 
• 115481-75-9 pyridinium 2-benzenesulfonate 
• 147510-56-3 Acetic acid 4-[(4-acetoxy-phenyl)-(2-chlorosulfonyl-phenyl)-methyl]-phenyl ester 
• 115511-92-7 bis(4.acetoxyphenyl)<2-(phenoxysulfonyl)phenyl>methane 
• 115481-73-7 Bis(phenol) phenyl sulfonate 
• 172464-36-7 2-(4,4'-dihydroxy-benzhydryl)-benzenesulfonic acid