6262-21-1

Basic information

• Product Name: 3,4,5,6-TETRACHLOROFLUORESCEIN
• Synonyms: 3,6-Dihydroxy-4',5',6',7'-tetrachlorospiro[9H-xanthene-9,1'(3'H)-isobenzofuran]-3'-one;3',6'-Dihydroxy-4,5,6,7-tetrachlorospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one;Einecs 228-413-3;Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 4,5,6,7-tetrachloro-3',6'-dihydroxy-;TETRACHLOROFLUORESCEIN;4,5,6,7-TETRACHLOROFLUORESCEIN;3,4,5,6-TETRACHLOROFLUORESCEIN;9’-(9H)xanthen)-3-one,4,5,6,7-tetrachloro-3’,6’-dihydroxy-Spiro(isobenzofuran-1(3H)
• CAS NO: 6262-21-1
• Molecular Formula: C₂₀H₈Cl₄O₅
• Molecular Weight: 470.09
• EINECS: 228-413-3
• Product Categories: Stains and Dyes;Stains&Dyes, A to;T-U-V
• Mol File: 6262-21-1.mol
• Article Data: 4

Chemical Properties

• Melting Point: 304℃
• Boiling Point: 752.3 °C at 760 mmHg
• Flash Point: 408.8 °C
• Appearance: /
• Density: 1.87 g/cm³
• Vapor Pressure: 4.23E-22mmHg at 25°C
• Refractive Index: 1.768
• CAS DataBase Reference: 3,4,5,6-TETRACHLOROFLUORESCEIN(CAS DataBase Reference)
• NIST Chemistry Reference: 3,4,5,6-TETRACHLOROFLUORESCEIN(6262-21-1)
• EPA Substance Registry System: 3,4,5,6-TETRACHLOROFLUORESCEIN(6262-21-1)

Safety Data

• WGK Germany: 3
• TSCA: Yes
• Hazardous Substances Data: 6262-21-1(Hazardous Substances Data)

Usage

General Description

3,4,5,6-Tetrachlorofluorescein is a chemical compound with the molecular formula C20H8Cl4O5. It is a synthetic fluorescent dye used in various applications such as in biological and medical research, as well as in forensics. The compound is known for its bright yellow-green fluorescence and is commonly used as a tracer dye in various imaging techniques. 3,4,5,6-Tetrachlorofluorescein is also used as a pH indicator and in the production of colored plastics. However, it is important to handle this compound with caution as it is toxic and should only be used in a well-ventilated area by trained professionals.

InChI:InChI=1/C20H8Cl4O5/c21-16-14(15(20(27)28)17(22)19(24)18(16)23)13-9-3-1-7(25)5-11(9)29-12-6-8(26)2-4-10(12)13/h1-6,25H,(H,27,28)

Upstream product

• 117-08-8 tetrachlorophthalic anhydride 
• 108-46-3 recorcinol 
• 85-44-9 phthalic anhydride 

Downstream Products

• 4159-77-7 Rose Bengal lactone 

Relevant articles and documents

Ti(IV) doping: An effective strategy to boost Lewis acidic performance of ZnO catalyst in fluorescein dye synthesis

Zn1-xTixO NPs were efficiently synthesized using a simple solution free mechanochemical method. The synthesized ZnO and Ti(IV)-doped ZnO catalysts are exhibited polycrystallinity, a hexagonal crystal structure, and roughly spherical agglomerates. The surface areas of the Zn1-xTixO catalysts were positively correlated with the doping percentage of Ti(IV), which also enhanced the Lewis acidity of the NPs. The catalysts exhibited excellent activity during the synthesis of fluorescein dyes. This methodology was also extended to sulfone-fluorescein dye synthesis.

PROCESS FOR THE SYNTHESIS OF 4,5,6,7-TETRACHLORO-3',6'-DIHYDROXY-2',4',5'7'-TETRAIODO-3H-SPIRO[ISOBENZOFURAN-1,9'-XANTHEN]-3-ONE (ROSE BENGAL) AND RELATED XANTHENES

A new process for the manufacture of iodinated xanthenes in high purity includes a cyclization step followed by an iodination step. No extraction, chromatographic or solvent concentration steps are required, and the intermediate as well as final compounds are isolated via filtration or similar means. The process requires a single organic solvent, and the steps are completed at temperatures below 100° C. The exclusion of chloride ions, of chloride free-radicals, hypochlorite ions, or hypochlorous acid as reagents or from reagents that may generate these species in situ in the presence of oxidants, prevents undesirable impurity formation. Several new compounds have been conceived and isolated using these methods. These new compounds are also formed into new medicaments.