651734-12-2

Basic information

• Product Name: 20S-Hydroxyvitamin D₃
• Synonyms: 20S-Hydroxyvitamin D₃
• CAS NO: 651734-12-2
• Molecular Weight: 400.645
• Mol File: 651734-12-2.mol
• Article Data: 5

Chemical Properties

• CAS DataBase Reference: 20S-Hydroxyvitamin D₃(CAS DataBase Reference)
• NIST Chemistry Reference: 20S-Hydroxyvitamin D₃(651734-12-2)
• EPA Substance Registry System: 20S-Hydroxyvitamin D₃(651734-12-2)

Safety Data

• Hazardous Substances Data: 651734-12-2(Hazardous Substances Data)

Upstream product

• 300344-51-8 1-((1S,3aR,4S,7aS)-4-Hydroxy-7a-methyl-octahydro-inden-1-yl)-ethanone 
• 189102-57-6 des-A,B-8β-[(triethylsilyl)oxy]-pregnane-20-one 
• 186372-30-5 (1R,3aR,4S,7aR)-octahydro-1-[(1’S)-1’-methyl-2’-oxo-ethanyl]-7a-methyl-4-triethylsilyloxy-1H-indene 
• 131852-63-6 (5Z,22E)-(7R)-3β-(tert-butyldimethylsilyloxy)-9,10-seco-8α-ergosta-5,10(19),22-triene-7,8-diol 
• 100858-27-3 (S)-(Z)-{2-[5-(tert-butyldimethylsiloxy)-2-methylenecyclohexylidene]ethyl}diphenylphosphine oxide 
• 67-97-0 vitamin D₃ 
• 1252023-07-6 C₂₉H₄₆O₃ 
• 154314-43-9 (1S,4S,7aS)-1-cetyl-7a-methyloctahydro-1H-inden-4-yl acetate 
• 101262-49-1 <1R-<1α(S^*),3aβ,4α,7aα>>-4-acetoxyoctahydro-α,7a-dimethyl-1H-indene-1-acetaldehyde 
• 172227-33-7 <1R-(1α,3aβ,4α,7aα)>-4-acetoxyoctahydro-1-<(1R,2E,4R)-1,4,5-trimethyl-2-hexenyl>-7a-methyl-1H-indene 
• 96685-53-9 (2Z)-2-<(5S)-5-(tert-butyldimethylsilyloxy)-2-methylene-cyclohexylidene>-ethanol 
• 1014717-75-9 (S,Z)-tert-butyl(2-(5-((tert-butyldimethylsilyl)oxy)-2-methylenecyclohexylidene)ethoxy)dimethylsilane 
• 67337-15-9 <(1Z),(5S)>-2-<5-hydroxy-2-methylcyclohexylidene>-ethanol 
• 84985-78-4 (4R,7aR)-1-((2R,5R,E)-5,6-dimethylhept-3-en-2-yl)-4-((R,Z)-1-hydroxy-2-((S)-5-hydroxy-2-methylenecyclohexylidene)ethyl)-7a-methyloctahydro-1H-inden-4-ol 

Downstream Products

• 1402226-61-2 1α,20,24-trihydroxyvitamin D₃ 
• 158946-37-3 1α,20,25-trihydroxyvitamin D₃ 
• 158946-41-9 1alpha,20-Dihydroxyvitamin D₃ 
• 1372617-13-4 20,25-dihydroxyvitamin D₃ 
• 1402226-50-9 20,24-dihydroxyvitamin D₃ 
• 1372617-14-5 20,26-dihydroxyvitamin D₃ 

Relevant articles and documents

Chemical synthesis, biological activities and action on nuclear receptors of 20S(OH)D3, 20S,25(OH)2D3, 20S,23S(OH)2D3 and 20S,23R(OH)2D3

New and more efficient routes of chemical synthesis of vitamin D3 (D3) hydroxy (OH) metabolites, including 20S(OH)D3, 20S,23S(OH)2D3 and 20S,25(OH)2D3, that are endogenously prod

Metabolism of 20-hydroxyvitamin D3 and 20,23-dihydroxyvitamin D3 by rat and human CYP24A1

CYP11A1 hydroxylates vitamin D3 producing 20S-hydroxyvitamin D3 [20(OH)D3] and 20S,23-dihydroxyvitamin D3 [20,23(OH)2D3] as the major and most characterized metabolites. Both display immuno-regulatory and anti-cancer properties while being non-calcemic. A previous study indicated 20(OH)D3 can be metabolized by rat CYP24A1 to products including 20S,24-dihydroxyvitamin D3 [20,24(OH)2D3] and 20S,25-dihydroxyvitamin D3, with both producing greater inhibition of melanoma colony formation than 20(OH)D3. The aim of this study was to characterize the ability of rat and human CYP24A1 to metabolize 20(OH)D3 and 20,23(OH)2D3. Both isoforms metabolized 20(OH)D3 to the same dihydroxyvitamin D species with no secondary metabolites being observed. Hydroxylation at C24 produced both enantiomers of 20,24(OH)2D3. For rat CYP24A1 the preferred initial site of hydroxylation was at C24 whereas the human enzyme preferred C25. 20,23(OH)2D3 was initially metabolized to 20S,23,24-trihydroxyvitamin D3 and 20S,23,25-trihydroxyvitamin D3 by rat and human CYP24A1 as determined by NMR, with both isoforms showing a preference for initial hydroxylation at C25. CYP24A1 was able to further oxidize these metabolites in a series of reactions which included the cleavage of C23-C24 bond, as indicated by high resolution mass spectrometry of the products, analogous to the catabolism of 1,25(OH)2D3 via the C24-oxidation pathway. Similar catalytic efficiencies were observed for the metabolism of 20(OH)D3 and 20,23(OH)2D3 by human CYP24A1 and were lower than for the metabolism of 1,25(OH)2D3. We conclude that rat and human CYP24A1 metabolizes 20(OH)D3 producing only dihydroxyvitamin D3 species as products which retain biological activity, whereas 20,23(OH)2D3 undergoes multiple oxidations which include cleavage of the side chain.

Chemical synthesis of 20S-hydroxyvitamin D3, which shows antiproliferative activity

20S-hydroxyvitamin D3 (20S-(OH)D3), an in vitro product of vitamin D3 metabolism by the cytochrome P450scc, was recently isolated, identified and shown to possess antiproliferative activity without inducing hypercalcemia. The enzymatic production of 20S-(OH)D3 is tedious, expensive, and cannot meet the requirements for extensive chemical and biological studies. Here we report for the first time the chemical synthesis of 20S-(OH)D3 which exhibited biological properties characteristic of the P450scc-generated compound. Specifically, it was hydroxylated to 20,23-dihydroxyvitamin D3 and 17,20-dihydroxyvitamin D3 by P450scc and was converted to 1α,20-dihydroxyvitamin D3 by CYP27B1. It inhibited proliferation of human epidermal keratinocytes with lower potency than 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) in normal epidermal human keratinocytes, but with equal potency in immortalized HaCaT keratinocytes. It also stimulated VDR gene expression with similar potency to 1,25(OH)2D3, and stimulated involucrin (a marker of differentiation) and CYP24 gene expression, showing a lower potency for the latter gene than 1,25(OH)2D3. Testing performed with hamster melanoma cells demonstrated a dose-dependent inhibition of cell proliferation and colony forming capabilities similar or more pronounced than those of 1,25(OH)2D3. Thus, we have developed a chemical method for the synthesis of 20S-(OH)D3, which will allow the preparation of a series of 20S-(OH)D3 analogs to study structure-activity relationships to further optimize this class of compound for therapeutic use.