MCC950 Sodium (CP-456773 Sodium)

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0 Metric Ton	Negotiable

0 Metric Ton

Negotiable

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Purity: >97%

Keywords

MCC950 Sodium (CP-456773 Sodium) CRID3 sodium salt 256373-96-3

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Biological Activity

Description

MCC950 Sodium (CP-456773 Sodium, CRID3 sodium salt) is a potent, selective inhibitor of NLRP3 with IC50 of 7.5 nM in BMDMs; but not the AIM2, NLRC4 or NLRP1 inflammasomes.

Targets

NLRP3 [1] (in BMDM (bone marrow derived macrophages))	NLRP3 [1] (in HMDM)
7.5nM	8.1 nM

In vitro

MCC950 inhibits canonical and noncanonical NLRP3 activation in BMDM and HMDM cells at IC50 of 7.5 and 8.1nM. MCC950 specifically inhibits activation of NLRP3 but not the AIM2, NLRC4 or NLRP1 inflammasomes.[1]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
mouse BMDM cells	Function assay		30 mins	Inhibition of NLRP3 in mouse BMDM cells preincubated for 30 mins followed by ATP addition by ELISA analysis. IC50=0.0075 μM	26422006
mouse BMDM cells	Function assay		30 mins	Inhibition of NLRP3 inflammasome activation in LPS-stimulated mouse BMDM assessed as reduction in NLRP3 inflammasome-induced IL-1beta production preincubated for 30 mins followed by NLRP3 stimulation with ATP by ELISA. IC50=0.0075 μM	27994733
INS-1 cells	Function assay	0.01 μM	1 h	MCC950 inhibited not only inflammasome activation but also Ang II-induced IL-1β elevation and cell apoptosis in INS-1 cells.	30939192

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Assay

Methods	Test Index	PMID
Western blot	NLRP3 / Casp-1 / BMI1 / ALDH1 / CD44;	28865486
Growth inhibition assay	Cell viability;	27670879
Immunofluorescence	ALDH1 / BIM1 / CD44;	28865486
ELISA	IL-1β;	28726778

In vivo

MCC950 reduces interleukin-1β (IL-1β) production and attenuates the severity of experimental autoimmune encephalomyelitis (EAE). Furthermore, MCC950 treatment rescues neonatal lethality in a mouse model of CAPS and is active in ex vivo samples from individuals with Muckle-Wells syndrome. [1]

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