ZCL 278

Min.Order / FOB Price:Get Latest Price

100 Gram	FOB Price: USD 1.0000

100 Gram

FOB Price: USD 1.0000

Min.Order :100 Gram
Purity: 98%
Payment Terms : L/C

Keywords

ZCL 278 2-(4-broMo-2-chlorophenoxy)-N-(4-(N-(4,6-diMethylpyriMidin-2-yl)sulfaMoyl)phenylcarbaMothioyl)acetaMide;ZCL 278;2-(4-Bromo-2-chlorophenoxy)-N-[[[4-[[(4,6-dimethyl-2-pyrimidinyl)amino]sulfonyl]phenyl]a 587841-73-4

Quick Details

Appearance:off white powder
Application:CAS：587841-73-4； Small molecule inhibitors, natural products
PackAge:100g,500g,1kg,25kg
ProductionCapacity:1000|Gram|Month
Storage:Dry seal
Transportation:shipping

Superiority:

We are specialized in custom synthesis, chemical/pharmaceutical/ pesticides outsourcing and contract research.

We are committed to provide excellence in researching, manufacturing and drug discovery process.

Our research team of scientists consists of western-trained Ph.D.s with experience and capabilities in drug R&D methodologies and medicinal chemistry.

Details:

ZCL278 is a Cdc42 small molecule modulator that directly binds to Cdc42 (Kd=11.4 uM) and inhibits Cdc42-intersectin interaction.
IC50 Value: 11.4 uM (Kd, SPR experiment) [1]
Target: Cdc42
in vitro: As ZCL278 has a weak absorption peak at 310 nm, to avoid any experimental error that might result from potential fluorescence quenching by ZCL278, the fluorescence emission of Cdc42 was monitored at 350 nm, at which ZCL278 has a negligible absorption. Thus, a Kd value of 6.4 μM was obtained. To further demonstrate the direct interaction between ZCL278 and Cdc42, a surface plasmon resonance (SPR) experiment was performed by covalently immobilizing purified Cdc42 onto CM5 chips and varying ZCL278 concentration. The SPR response was observed to increase along with elevated ZCL278 concentrations, and eventually gave a Kd of 11.4 μM [1]. The experimental pKa values of ZCL278 were determined to be 3.48 ± 0.04, 6.61 ± 0.02, and 7.45 ± 0.01, the pKa value of 3.48 is associated to pyrimidine nitrogen that should stay in a neutral form at pH 7.4 [1]. ZCL278-induced disruption of perinuclear distribution of active Cdc42 reflected its effects on Golgi organization, we examined GM130, a peripheral cytoplasmic protein that is tightly bound to Golgi membranes and helps to maintain cis-Golgi structures.

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