Name: IPTG, Isopropyl-Beta-D-1-thiogalactopyranoside Description: IPTG is used in conjunction with X-Gal, Product # X-1015, in the Blue/White colon…
Name: IPTG, Isopropyl-Beta-D-1-thiogalactopyranoside Description: IPTG is used in conjunction with X-Gal, Product # X-1015, in the Blue/White colony screening assay. X-Gal is metabolized by the enzyme, beta-galactosidase, and it produces a by product this is a bright blue color. Beta-galactosidase is coded for in the LacZ gene. This LacZ gene also provides multiple cloning sites where the plasmid may be cut and DNA may be added. These two points prove ideal for the scientist, The scientist now only needs to induce expression of the LacZ gene. That turns out to be the inducer, IPTG, Product I-1312. The scientist now has a manner to clearly identify cells that have incorporated his/her gene of interest.. Chemical Formula: C9H18O5S Molecular Weight: M.W. 238.3 Solubility: Clear and colorless solution (Sol. 5%, H2O). CAS Number: CAS# [367-93-1] Active Product: Yes Appearance: White crystalline powder. Purity: >99% HPLC Handling: Store in Tightly Sealed Vial. Storage: Room Temperature. Shipping: Priority Courier
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