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HEPES Sodium salt price 75277-39-3 supplier HEPES-Na manufacture
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We own manufacture bases in Shandong,Zhejiang and other places. We have a professional, efficient sales team. Our R & D strength is strong and we cooperate with a number of colleges and universities at home and abroad long-term .80 percent of our products are exported. We have experience of mansy years in biochemical ,electronic chemical field and have a good reputation.
Under strict quality control of the production of pharmaceutical raw materials in accordance with the GMP standard ICH Q7A, we passed the ISO9001 quality system assessment in 2002,we always have professionalism, integrity, innovation, cooperation of the business philosophy in the domestic and international customers services.
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English Name:[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Na
Molecular Formula:C8H17N2O4SNa
Molecular Wt:260.3
Appearance:White powder
Assay:99.55%
Enterprise Standard
Application: it is an important PH stable reagent in biotic experiments.
Appropriate PH is obtained by mixing mild acid and its conjugate base.
Many biological responses happen in neutral condition (usually PH 6-8),
so the buffering range is required to be in PH 6-8. In addition, the form
of acid-base of buffer should not chelate with some metallic ions.
Characteristics of biological buffer:
1, Strong water-solubility;
2. Compatibility of the acid-base dissociation balance;
3. Low membrance penetration rate;
4. Low metal chelating ability;
5. High chemical stability;
6. Low absorption in ultraviolet and visible region.
HEPES Sodium salt (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt) may be used in the preparation of HEPES buffer, which has been described as one of the best all-purpose buffers available for use in biological research. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5.
Usage
• Recommended working concentration: 10-25 mM
• A buffer solution of HEPES can be prepared by any of several methods. The free acid can be added to water, then titrated with sodium hydroxide or potassium hydroxide to the pH desired. Alternatively, equimolar concentrations of HEPES (sc-29097) and of sodium HEPES can be mixed in approximately equal volumes, back-titrating with either solution to the appropriate pH.
• This product is a high quality reagent, but has not been evaluated for use in cell culture. Please employ appropriate contaminant testing and/or sterilization techniques for the intended application.
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