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5 Gram |
FOB Price:USD 30.0000 -60.0000 |
INT manufacture 146-68-9 supplier Iodonitrotetrazolium chloride price
Best quality with best price.
ISO Certificate
Our main market is America,Europe,and Asia.We have some famous clients like MERCK,Bristol-Myers Squibb,DOW,SANYO,Fisher Scientific,Sigma-ALDRICH
We own manufacture bases in Shandong,Zhejiang and other places. We have a professional, efficient sales team. Our R & D strength is strong and we cooperate with a number of colleges and universities at home and abroad long-term .80 percent of our products are exported. We have experience of mansy years in biochemical ,electronic chemical field and have a good reputation.
Under strict quality control of the production of pharmaceutical raw materials in accordance with the GMP standard ICH Q7A, we passed the ISO9001 quality system assessment in 2002,we always have professionalism, integrity, innovation, cooperation of the business philosophy in the domestic and international customers services.
The center has advanced instruments and equipments from domestic and abroad. Now there are 2 doctors who major in biology with oversea educational background, 3 masters who major in medicine and organic synthesis, several engineers. The center periodically keeps communications with the colleagues from domestic and abroad to know the latest information of scholarly research. The center mainly dedicates to the research on biomedicine and biochemistry. The present products, such as fluorescent label reagent, color development reagent, dye reagent, biological buffer, enzyme for diagnosis, are very welcomed by the clients from domestic and abroad.
Product ID: TQDR020
Name: INT
Specification/price: 10g,50g,100g
Rank: Ultra Pure Grade
CAS: 146-68-9
English Name: 2-(4-iodophenyl)-3-(4-nitrophrnyl)-5-phenyl-2h-tetrazoliu chloride
Molecular Formula:C19H13ClIN5O2
Molecular Wt:505.7
Appearance:pale yellow or pale yellowish orange powder
Assay:98.2%
Enterprise Standard
Usage of INT: used to detect dehydrogenase’s electron acceptor in chromatometry
Description
Iodonitrotetrazolium chloride is an electron acceptor used in various colorimetric assays. The chemical has been mainly documented for its measurement of dehydrogenase activity. In the assay to measure SDH (succinate dehydrogenase) activity, Iodonitrotetrazolium chloride was used as an oxidizing agent being reduced by succinate dehydrogenase. Upon reduction the compound forms a formazan dye measured at 500 nm. Iodonitrotetrazolium chloride has also been used to study the electron transport activity in soils, sediments, and pure enzyme cultures as well as nucleotide diphosphate kinase activity.
Usage
spectrophotometric assay for succinate dehydrogenase activity: Materials: spectrophotometer cuvette with 1-cm light patch. Reaction Mixture: 100 mmol/liter triethanolamine (HCl), pH8,3 o.5 mmol/liter EDTA, 2 mmol/liter Iodonitrotetrazolium chloride, 12 g/liter Cremophor EL, 20 mmol/liter succinate, pH7.4 Reaction: mix 990 ul of reaction mixture with 10ul mitochondrial suspension color develops at 30ºC record for 6 min. @500 nm
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