Oligosaccharide industrial Chitosan Oligosaccharide food Oligosaccharide industrial
dosage form Items |
sold dosage form |
liquid dosage form |
Appearance |
Tawny powder, Easily, dissolved in water(There may be a small amount of insoluble stabilizer that can be degradated by the enzyme) |
Tawny liquid |
Activity*(u/g u/ml) |
250unit/g |
10 unit/ml |
Loss on Drying |
≤8% |
|
Activity preservation rate(preservation for three months at 25℃) |
≥90% |
≥90% |
Heavy Metal (as pb) |
≤30mg/kg |
≤30mg/kg |
Arsenic |
≤3mg/kg |
≤3mg/kg |
coliform group |
≤3000cfu/100g |
≤3000cfu/100g |
GS-CHITOSANASE is a new type of industial enzyme preparation developed by the company. The enzyme preparation is mainly used for making high quality chitosan oligosaccharides (amino- oligosaccharins) produced by the method of specific enzymolysis degradation.
Unique feature
1. The strains producing the enzyme preparation is a Bacillus subtilis GF07 that does not produce toxin and is not pathogenic to humans, as a result it is safe to use.
2.The main active ingredient of GS-CHITOSANASE is chitosanase(EC 3.2.1.132)that is a enzyme with specificity endo-hydrolysis activity against chitosan and the exo-hydrolase activity of exo-β- glucosaminidase (EC 3.2.1.165)and β-N- acetyl-hexosaminidase(EC 3.2.1.52)has not yet detected in the enzyme preparation. As a result, GS-CHIYOSANASE is very suitable for the preparation of chitooligosaccharides.
3. The main enzymolysis products of GS-CHITOSANASE against chitosan are the chitosan oligosaccharides with degree of polymerization from 2 to 10.In which the relative yield of oligomer with DP (5-10) can be as high as 40-80%.
Use
1 GS-CHITOSANASE is very suitable for contral of the enzymolysis on chitosan to make some product of chitooligosaccharide with special polymerization degree and narrow molecular weight distribution.
2 GS-CHITOSANAE is also used to produce of water-soluble low-molecular-weight chitosan.
Examples of application
Example 1
Under normal circumstances ,the chitooligosaccharide ,obtained from chitosan hydrolysis more fully by GS-CHITOSANSE, is a product of average molecular weight 1000, dispersity 1.06 and polymerization degree distribution from 3 to 12.
① The test result of high-performance GPC of the product
HPGPC spectrum (determinated by College of Resource and Environmental Science, Wuhan University Chitin Research and Development Centre of Wuhan University)
Data of HPGPC
Weight-average molecular weight 1005.6
Number-average molecular weight 942.3
Molecular weight distribution of the product 1.0670
②The test result of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry of the product
Fig1 MALDI-TOF-MS (determinated by Beijing MS centre ,Institute of Chemistry Chinese Academy of Sciences )
Fig 2 Date analysis of MALDI-TOF-MS(the digital at the top of ion peak of chitosan oligomer in the spectrum show thepolymerization degree of the oligomer .)
Fig 3 The polymerization degree distribution and relative content of the chitooligosaccharides in the product
Fig 4 The relative content of homogeneous chitosan-oligosaccharides in the product
Example 2
The product, obtained from GS-CHITOSANSE limited hydrolysis against chitosan and grading separation according to molecular weight size of the oligomer, may be chitooligosaccharides of average molecular weight 2500, dispersity 1.15 and polymerization degree distribution from 6 to 9.
① The test result of high-performance GPC of the product
HPGPC spectrum (determinated by College of Resource and Environmental Science, Wuhan University Chitin Research and Development Centre of Wuhan University)
Data of HPGPC
Weight-average molecular weight 2352.8
Number-average molecular weight 2041.6
Molecular weight distribution of the product 1.15240
②The test result of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry of the product
Fig1 MALDI-TOF-MS (determinated by College of Life Science Hunan Normal University )
Fig 2 Date analysis of MALDI-TOF-MS(the digital at the top of ion peak of oligomer in the spectrum show the polymerization degree of the oligomer .)
Fig 3 The polymerization degree distribution and relative content of the chitooligosaccharides in the product
Example 3
An domestic enterprise small-lot produced chitooligosaccharides by using GS-CHITOSANASE, the testing results of matrix-assisted laser desorption ionization / time-of-flight mass spectrometry shows that all oligomer is uniform chitosan oligosaccharides with polymerization degree distribution from 2 to 9 , the relative content of chitohexaose is high 20% in the product.
Pig The polymerization degree distribution and relative content of the chitosan oligosaccharides in the product
Standard
dosage form Items |
sold dosage form |
liquid dosage form |
Appearance |
Tawny powder, Easily, dissolved in water(There may be a small amount of insoluble stabilizer that can be degradated by the enzyme) |
Tawny liquid |
Activity*(u/g u/ml) |
250unit/g |
10 unit/ml |
Loss on Drying |
≤8% |
|
Activity preservation rate(preservation for three months at 25℃) |
≥90% |
≥90% |
Heavy Metal (as pb) |
≤30mg/kg |
≤30mg/kg |
Arsenic |
≤3mg/kg |
≤3mg/kg |
coliform group |
≤3000cfu/100g |
≤3000cfu/100g |
* The measurement of GS-CHITOSANAE activity is based on the reducing amount of the chitosan substrate in unit time. One unit of chitosanase in the enzyme preparation is defined as the amount of enzyme required to transform 1mg of chitosan into water-soluble components in one min at 45℃,p, H 5.5.
About|Contact|Cas|Product Name|Molecular|Country|Encyclopedia
Message|New Cas|MSDS|Service|Advertisement|CAS DataBase|Article Data|Manufacturers | Chemical Catalog
©2008 LookChem.com,License: ICP
NO.:Zhejiang16009103
complaints:service@lookchem.com Desktop View