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Chloroprotoferrihem CAS no 16009-13-5
MF: C34H32ClFeN4O4
MW: 651.94
CAS: 16009-13-5
Assay: 99%
Appearance: dark purple crystalline powder
folding edit this paragraph Dark brown flaky crystal, insoluble in water and acetic acid, slightly soluble in 70% ~ 80% ethanol, soluble in dilute sodium hydroxide solution, in sodium hydroxide solution to generate hydroxyl high iron red element, organic alkali soluble in strong (such as trimethylamine and dimethyl aniline) and acetic acid, soluble in concentrated sulfuric acid and lose iron, soluble in dilute ammonia; Transmitted light brown, reflected light for steel blue, until 300 ℃ melting
ITEM |
SPECIFICATION |
RESULT |
Appearance: |
fine Powder |
Conformed |
Odor: |
Characteristic |
Conformed |
Taste: |
Characteristic |
Conformed |
Drying Method: |
Spary drying |
Conformed |
Loss on Drying: |
8.0% Max. |
4.35% |
Bulk Density: |
40~60g/100ml. |
49.5g/100ml |
Total Heavy Metals: |
10ppm Max. |
Conformed |
Arsenic: |
2ppm Max. |
Conformed |
Lead: |
2ppm Max. |
Conformed |
Total plate count: |
1,000cfu/g Max. |
Conformed |
Yeast&mold: |
100cfu/g Max. |
Conformed |
E.Coli: |
Negative |
Negative |
Salmonella: |
Negative |
Negative |
Staphylococcus: |
Negative |
Negative |
Conclusion: The product conforms to the standard. |
General Description |
Heme is a small molecule present either free or bound to hemoglobin in the bloodstream of mammals. This is an alternative source of iron within the host that contains a porphyrin ring containing a Fe2+ ion called hemin. |
Biochem/physiol Actions |
Hemin, the oxidized version of heme, is an iron-containing prosthetic group for a diverse group of proteins. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Free hemin levels may be upregulated in various pathological conditions and can contribute to various inflammatory conditions including vascular disorders, renal failure, and immune-mediated disorders.Hemin may be used to study the expression and represson of heme oxygenase 1 (HO-1) as well as the activity of HO-1. Hematin is the "X factor" required to grown Haemophilus influenzae. |
Purification Methods |
Hemin is purified by recrystallisation from AcOH. Also, hemin (5g) is shaken in pyridine (25mL) till it dissolves, then CHCl3 (40mL) is added, the container is stoppered and shaken for 5minutes (releasing the stopper occasionally). The solution is filtered under slight suction, and the flask and filter are washed with a little CHCl3 (15mL). During this period, AcOH (300mL) is heated to boiling, and saturated aqueous NaCl (5mL) and conc HCl (4mL) are added. The CHCl3 filtrate is poured in a steady stream, with stirring, into the hot AcOH mixture and set aside for 12hours. The crystals are filtered off, washed with 50% aqueous AcOH (50mL), H2O (100mL), EtOH (25mL), Et2O and dried in air. [Fischer Org Synth Coll Vol III 442 1955, Beilstein 26 III/IV 3048.] |
InChI:InChI=1/C34H34N4O4.ClH.Fe/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;;/h7-8,13-16H,1-2,9-12H2,3-6H3,(H4,35,36,37,38,39,40,41,42);1H;/q;;+3/p-3/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;;/rC34H32ClFeN4O4/c1-7-21-17(3)26-14-31-22(8-2)19(5)29-13-25-18(4)23(9-11-33(41)42)28(38-25)16-32-24(10-12-34(43)44)20(6)30(15-27(21)37-26)40(32)36(35)39(29)31/h7-8,13-16H,1-2,9-12H2,3-6H3,(H,41,42)(H,43,44)/b25-13-,26-14-,27-15-,28-16-,29-13-,30-15-,31-14-,32-16-
ProtoporphyrinIX-zinc oxide (PP-ZnO) nan...
-
iron(III) chloride
protoporphyrin IX
iron(III) protoporphyrin IX chloride
Conditions | Yield |
---|---|
In water; dimethyl sulfoxide; for 12h;
|
L-glutamic acid
(SP-5-13)-chloro(7,12-diethenyl-3,8,13,17-tetramethyl-21H,23H-porphine-2,18-dipropanoato(4-)-N21,N22,N23,N24)-ferrate(2-), dihydrogen
Conditions | Yield |
---|---|
for 24h; radioactivity investigations with duck blood;
|
L-glutamic acid
protoporphyrin IX
heme b
ferriprotoporphyrin IX hydroxide
protoporphyrin IX dimethyl ester
biliverdin IXγ dimethyl ester
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