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Negotiable |
Isopropyl-beta-D-thiogalactopyranoside 99%
Appearance | White to almost white crystalline powder |
Purity (By HPLC) |
Min 99%+ |
Assay by HPLC |
98% - 101% (On Anhydrous Basis) |
IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of thelacoperon and induces expression of β-galactosidase inEscherichia coli.The compound is widely used with X-Gal plates to screen for blue and white colonies. Thelacoperon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity.
Product Name | Isopropyl-beta-D-thiogalactopyranoside |
Synonyms | IPTG;Isopropyl-beta-D-thiogalactoside;IPTG, HEMIDIOXANE ADDUCT;ISOPROPYL-1-THIO-BETA-D-GALACTOPYRANOSIDE;ISOPROPYLTHIO-BETA-GALACTOSIDE;ISOPROPYLTHIO-BETA-THIOGALACTOSIDASE;ISOPROPYLTHIO-B-THIOGALACTOPYRANOSIDE;ISOPROPYL THIOGALACTOSIDE;ISOPROPYL-BETA-D-THIOGALACTOPYRANOSIDE, DIOXANE FREE;Isopropyl β-D-1 Thiogalactopyranoside;propan-2-yl 1-thio-beta-D-galactopyranoside;1-methylethyl 1-thio-D-galactopyranoside |
Molecular Formula | C9H18O5S |
Molecular Weight | 238.3012 |
InChl | InChI=1/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8- ,9?/m1/s1 |
CAS Registry Number | 367-93-1 |
Molecular Structure |
|
Appearance | White to almost white crystalline powder |
Purity (By HPLC) |
Min 99%+ |
Assay by HPLC |
98% - 101% (On Anhydrous Basis) |
Specific optical rotation |
-31.5 ± 3.0° [a]D20(c=1,H2O) |
Melting Point | 110-114℃ |
Boiling Point | 438.4°C at 760 mmHg |
pH |
5.0 - 7.0 |
Dioxane free | Not detected |
Solubility | Soluble in water, and methanol. |
Water content (BY KF) | Max 1.0% |
Storage | Shipped at Ambient Temperature. Store at +2 - 8°C. |
Description |
IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of thelacoperon and induces expression of β-galactosidase inEscherichia coli.The compound is widely used with X-Gal plates to screen for blue and white colonies. Thelacoperon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity. |
Usage |
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Induces protein synthesis inE. coliwhen transcription is controlled by thelac-promoter. In conjunction with X-gal,q.v.,in detection oflacgene activity during cloning experiments.
IPTG is an inducer of β-galactosidase activity in E. coli. It is commonly used in conjunction with X-GAL to
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Application | An analogue of allolactose that binds specifically to the repressor protein of thelacoperon. |
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