Isopropyl-beta-D-th...

Isopropyl-beta-D-thiogalactopyranoside

Isopropyl-beta-D-thiogalactopyranoside

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  • Purity: 98% - 101%

Keywords

Isopropyl-beta-D-thiogalactopyranoside 99%

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  • Appearance:White to almost white crystalline powder
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Superiority:

 

Appearance    White to almost white crystalline powder

 Purity (By HPLC)

  Min 99%+

Assay by HPLC

  98% - 101%   (On Anhydrous Basis) 

 

Details:

IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of thelacoperon and induces expression of β-galactosidase inEscherichia coli.The compound is widely used with X-Gal  plates to screen for blue and white colonies. Thelacoperon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity.
 

 

Product Name Isopropyl-beta-D-thiogalactopyranoside
Synonyms IPTG;Isopropyl-beta-D-thiogalactoside;IPTG, HEMIDIOXANE ADDUCT;ISOPROPYL-1-THIO-BETA-D-GALACTOPYRANOSIDE;ISOPROPYLTHIO-BETA-GALACTOSIDE;ISOPROPYLTHIO-BETA-THIOGALACTOSIDASE;ISOPROPYLTHIO-B-THIOGALACTOPYRANOSIDE;ISOPROPYL THIOGALACTOSIDE;ISOPROPYL-BETA-D-THIOGALACTOPYRANOSIDE, DIOXANE FREE;Isopropyl β-D-1 Thiogalactopyranoside;propan-2-yl 1-thio-beta-D-galactopyranoside;1-methylethyl 1-thio-D-galactopyranoside
Molecular Formula   C9H18O5S
Molecular Weight   238.3012
InChl   InChI=1/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-   ,9?/m1/s1
CAS Registry Number   367-93-1
   
Molecular Structure                  294337 1
   
Appearance    White to almost white crystalline powder

 Purity (By HPLC)

  Min 99%+

Assay by HPLC

  98% - 101%   (On Anhydrous Basis) 

Specific optical rotation

  -31.5 ± 3.0°  [a]D20(c=1,H2O)
Melting Point   110-114℃
Boiling Point   438.4°C at 760 mmHg

pH
(5% in water)

  5.0 - 7.0
Dioxane free   Not detected
Solubility   Soluble in water, and methanol.
Water content              (BY KF)   Max 1.0%
 Storage   Shipped at Ambient Temperature. Store at +2 - 8°C.
Description  IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of thelacoperon and induces expression of β-galactosidase inEscherichia coli.The compound is widely used with X-Gal  plates to screen for blue and white colonies. Thelacoperon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity.
 
 Usage

 Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Induces protein synthesis inE. coliwhen transcription is controlled by thelac-promoter. In conjunction with X-gal,q.v.,in detection oflacgene activity during cloning experiments.

 

IPTG is an inducer of β-galactosidase activity in E. coli. It is commonly used in conjunction with X-GAL to
detect lac gene expression in cloning applications, thus allowing dection of recombinant molecules.

 

   
Application                                              An analogue of allolactose that binds specifically to the repressor protein of thelacoperon.

 

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