Recombinant Human Keratinocyte Growth Factor-2 (rhKGF-2)
1.Description:
Keratinocyte Growth Factor-2 (KGF-2) was originally identified from rat embryos by
homology-based polymerase chain reaction. Human and mouse KGF-2 were subsequently cloned. The human KGF-2 cDNA encodes a 208 amino acid residue protein with a hydrophobic amino-terminal signal peptide. Human KGF-2 shares approximately 92% and 95% amino acid sequence identity with mouse and rat KGF-2, respectively. Among the FGF family members, KGF-2 is most closely related to FGF-7. KGF-2 transcripts has been shown to be most abundant in the embryo and adult lungs. Recombinant KGF-2 preparations have been shown to be mitogenic for epithelial and epidermal cells but not fibroblasts. Based on its in vitro biological activities and in vivo expression pattern, KGF-2 has been proposed to play unique roles in the brain, in lung development, wound healing and limb bud formation.
Synonyms:FGF-10, FGF10
Grade: cosmetic
AA Sequence: MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY
FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG
KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR
GQKTRRKNTS AHFLPMVVHS
Source: Escherichia coli
Molecular Weight: Approximately 19.3 kDa, 170 amino acid residues consisting of Methionine and the mature human KGF-2 (amino acid residues 40 – 208).
Purity:>95% by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active. The ED50 is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106 units/mg, as determined by proliferation of BaF3 cells expressing FGF receptors.
Physical Appearance: White lyophilized powder.
Formulation: Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin < 1EU/ug of growth factor as determined by LAL method.
2.Reconstitution: Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
3.Storage: Store at -200C after receiving. Upon reconstitution, store at 2-80C for up to one week. For maximal stability, aliquot and store at -20?C. Avoid repeated freeze/ thaw cycles.
4.Usage:This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.