9011-93-2 LYSOSTAPHIN LYSOSTAPHIN Price
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From Staphylococcus staphylolyticus
Storage Temperature: –20 oC or 2~8℃
CAS No. : 9011-93-2
Synonym: Glycyl-glycine Endopeptidase
Product Description
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against Staphylococcus species, including S. aureus.1,2 Lysostaphin has hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall of Staphylococcus species, which leads to cell lysis.3,4
Purified extracellular lysostaphin from S. staphylolyticus is a single polypeptide chain with a molecular mass of 26,926 Da, containing 246 amino acid residues.5 Lysostaphin has an isoelectric point of 9.5 and a pH optimum of 7.5.6
The product is a lyophilized powder containing 50–70% protein with the balance primarily as NaCl.
Specific Activity: ≥1200 units/mg protein
Unit Definition: One unit will reduce the turbidity (A620) of a suspension of S. aureus cells from 0.250 to 0.125 in 10 minutes at pH 7.5 at 37 oC in a 6.0 mL reaction mixture.
Precautions and Disclaimer
This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Preparation Instructions
This product is soluble in water (10 mg/mL), yielding a clear to slightly hazy solution. We recommended that fresh solutions be prepared, as the product loses activity in solution. We do not specifically recommend the freezing of lysostaphin solutions in aliquots. One published reference cites storage of 200 units/mL stock solutions of lysostaphin in a buffer of 0.05 M Tris-HCl and 0.145 M NaCl at pH 7.4.7 A second reference indicates storage of 2 mg/mL stock solutions in 20 mM sodium acetate buffer, pH 4.5, in frozen aliquots.8 However, we have not tested either situation ourselves.
Application
Degrade the bacteria which have a glycine peptide bond structure in the cell wall such as Staphylococcus aureus, to lyse the cell wall to achieve the purpose of sterilization.
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