EGF epidermal growth factor supplier epidermal growth factor factory
★ Product Description
Recombinant Human Epidermal Growth Factor (EGF) is a biologically active protein factor for cell culture. It has a profound effect on the differentiation of specific cells in vivo, and is a powerful mitotic factor for a variety of ectoderm and mesoderm cells cultured in vitro.
This product is a lyophilized powder prepared by recombinant yeast, freeze-dried by multi-step separation and purification, and can be widely used in cell culture.
★ Product features
l No animal origin: the product is free of animal virus, no pathogenic substances, no external factor pollution, and high safety.
l High purity: SDS-PAGE electrophoresis purity reaches 98%.
l High specific activity: Biological activity per 1mg protein is 1.2×106U~2.0×106U. Small amount of protein can achieve the desired experimental results.
l Quality stability: This product is a lyophilized powder containing bioactive protective agent. It has been mass-produced and the difference between batches is small.
★ Product Properties
Type |
Human source |
Expression system |
Pichia pastoris |
Appearance |
White or off-white powder |
Molecular weight |
6KD±0.6KD |
purity |
≥98%(SDS-PAGE) |
≥98%(HPLC) |
|
Endotoxin |
< 1 EU/µg |
Specific activity |
Reachable 2×106 U/mg pro. |
Biological activity: According to Chinese pharmacopoeia 2015 edition,it can use EGF to stimulate BALB/3T3 to test the biological activity .
★ Product Application
It can be used for cell culture to enhance cell proliferation, cell growth and cell differentiation.
★ Usage method
l It is recommended to centrifuge the powder to the bottom of the tube before opening the lid, then dissolve it in sterilized deionized water to prepare a storage solution of 100μg/ml. It can be stored stably for 2 weeks at 2°C~8°C. When diluted to a lower concentration (not less than 10 μg/ml), 0.1% BSA or HSA is required. If it is necessary to extend the storage time, it is recommended to freeze at -70 ° C or -20 ° C. It is not recommended to repeat freezing and thawing.
l This product is for research use only. Not for animals and humans
★ Transportation and storage Stability
l Transport stability:It is transported in an ice pack to keep it active.
l Storage stability:It is stored at -15 ° C to -20 ° C and can be stable for 24 months..
★ Related products
Recombinant Human Basic Fibroblast Growth Factor (bFGF);
Recombinant Human Insulin-like Growth Factor-1 (IGF-1).
★ Product specifications
Item number |
specification |
P901-100μg |
100μg/ Vial |
P901-500μg |
500μg/Vial |
P901-1mg |
1mg/ Vial |
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