Triamcinolone Acetonide CAS Rn 76-25-5

Superiority:

use
Corticosteroids, acting on glucose metabolism, can reduce the antibody tissue damage caused by the pathological response, for active rheumatism and rheumatoid arthritis.
Measurement method editor
Method name: triamcinolone API - triamcinolone acetonide - high performance liquid chromatography
Applications: This method uses high performance liquid chromatography Determination of triamcinolone acetonide content.
This method is suitable for triamcinolone API.
Principle of the method: the test sample was dissolved in methanol and quantitatively diluted, into the high performance liquid chromatography for chromatographic separation, with UV absorption detector at a wavelength of 240nm at the detection of triamcinolone peak area, calculate its content.
Reagents: methanol
Equipment: 1. Instruments
1.1 high performance liquid chromatography
1.2 column
Octadecylsilane bonded silica as a filler, the theoretical number of plates calculated by triamcinolone peak should be not less than 5000.
1.3 UV absorption detector
2. Chromatographic conditions
2.1 Mobile phase: Methanol water = 525 475
2.2 Detection wavelength: 240nm
2.3 column temperature: room temperature
Sample preparation:
1. Preparation of reference solution
Accurately weighed amount of triamcinolone acetonide reference substance, dissolved in methanol and quantitatively diluted into 1mL each containing about 30 & micro; g solution, which is the reference solution.
2. Preparation of the test solution
Accurately weighed for the right amount of the test substance, dissolved in methanol and quantitatively diluted into about 1mL each containing triamcinolone acetonide 30 & micro; g solution, that is for the test solution.
Note: "Precision weighed" means that weighed weight should be accurate to the weight of the said one thousandth. "Precision measurement" refers to the accuracy of measuring volume should be consistent with the national standard of precision pipette volume requirements.
Steps: Precision drawing the reference solution and the test solution of 20mL, into the high performance liquid chromatography with UV absorbance detector at a wavelength of 240nm at the determination of triamcinolone (C24H31FO6) peak area, calculate its content.
Pharmacopoeia standard editor
Source (name), content (potency)
This product is 9-fluoro-11β, 21-dihydroxy-16α, 17 [(1-methylethylidene) bis (oxy)] - pregna-1,4-diene-3,20-dione. Calculated on dry goods, containing C24H31FO6 should be 97.0% ~ 102.0%.
Traits
This product is white or white crystalline powder; odorless.
This product is dissolved in acetone, slightly soluble in chloroform, slightly soluble in methanol or ethanol, very slightly soluble in water.

Identification
(1) In the chromatogram recorded under the content determination, the retention time of the main peak of the test solution should be consistent with the retention time of the main peak of the reference solution.
(2) This product's infrared absorption spectrum should be consistent with the control map ("Medical Infrared Spectroscopy set" 603).

Assay
Take this product, accurately weighed, add methanol dissolved and quantitatively diluted solution made of about 30μg per 1ml, the precise amount of 20μl into the liquid chromatograph, record the chromatograms; the other triamcinolone reference substance, the same method . According to external standard peak area calculation, that is [3].
category
Cortina drugs.
Storage
Shading, sealed and preserved.
preparation
(1) Triamcinolone acetonide injection (2) Triamcinolone Econazole Cream