High quality Guanidine thiocyanate Cas 593-84-0 with best price

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High quality Guanidine thiocyanate Cas 593-84-0 with best price

CAS 593-84-0 China Wholesale Guanidine Thiocyanate 99.50%

CAS 593-84-0 China Wholesale Guanidine Thiocyanate 593-84-0
Wholesale Price Guanidine Thiocyanate CAS 593-84-0
Wholesale Price Bulk in Stock Guanidine Thiocyanate 

Specification

Items	Specification	Result
Appearance	White to slightly off-white crystalline powder	Complies
Assay	≥99.5%	99.83%
Melting point	118℃-121℃	120.3℃-120.9℃
Moisture	≤0.3%	0.21%
Ash	≤0.1%	0.007%

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Our package of product CAS 593-84-0 : 25kg/drum

Questions And Answer    Back Directory
[Description]
Guanidine thiocyanate is one of the cheapest and easiest to prepare of the guanidine salts. Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents. It is provided in one bottle containing 500 g. It is recommended for isolation of RNA, especially for tissues such as pancreas with high levels of RNase activity. In solution, it is known as guanidinium thiocyanate. Guanidine Thiocyanate is thoroughly tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
[Uses]

Guanidine thiocyanate is a potent protein denaturant (stronger than guanidine HCl) often used in the isolation of intact ribonucleic acid to eliminate RNase activity. RNase can recover activity after boiling, but is irreversibly inactivated in a 4 M solution of guanidine thiocyanate. Such solutions, to which the reducing agent b-mercaptoethanol is often added, are used to inactivate RNAse when isolating RNA from tissues that are rich in RNase, such as liver. Total nuclear and cytoplasmic RNA may be isolated this way. A protocol for extracting RNA with guanidine isothiocyanate has been published.
In the presence of guanidine thiocyanate, proteins dissolve readily, cellular structures disintegrate and nucleoproteins dissociate from nucleic acids, as protein secondary structure is lost.
[Biochem/physiol Actions]

Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction.

 

 

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      Wuhan Fortuna Chemical Co., Ltd, located in the predominant Wuhan Citywhere is a traffic hinge of China,is a big integrative chemical enterprise being engaged in producing and developing pharmaceutical & its intermediates, food additive  and plant extract, and in distributing the produc ts of our own company and affiliated enterprises.

     

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