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Creatine is a kind of natural nutrients in presented in the human body and can also be synthesize through arginine, glycine, and methionine in the liver, kidney and pancreas: in the presence of the catalysis of the arginine/glycine transamidinase in the kidney, the amidino group of the arginine can be transferred to the amino group of the glycine, generating glycocyamine. Then in the liver, with the catalysis of the glycocyamine methyltransferase, the methyl group of the S-adenosylmethionine can be transferred to the glycocyamine, generating creatine; it can increase the water content of the muscle cells, help the muscle cells to store energy, increase the protein synthesis and some other fundamental functions; wherein the artificially synthetic creatine can be used as the nutritional supplements for promoting the adaptation of skeletal muscle on fierce exercise and fighting against the excessive fatigue of individual in weakness; it can also be used for making the drugs for the treatment of heart disease and respiratory insufficiency; it can be used for making the pharmaceutical formulation containing human growth hormones; it can be applied to the compound health food, with anti-aging and physical power-recovering effect.

Creatine is involved in the rapid production of ATP associated with creatine kinases in skeletal muscle tissue. Creatine may be used as a supplement to study its mechanism of action. It acts as a nutraceutical and a neuroprotective agent.