How is heparin lithium produced

Details:

Heparin lithium is a commonly used anticoagulant, CAS 9045-22-1. It is white powder and is mainly used in blood

The effect of inspection is obvious.

Heparin lithium specification

Production process

It mainly takes crude heparin as raw material. After refining, the high-quality product is obtained by ion exchange with lithium salt product. however

After that, the enzyme is immobilized to enhance the activity of the enzyme, the enzymatic hydrolysis effect is more sufficient, and the impurities can be removed after simplification to improve the production efficiency

Greatly improved and reduced the production cost. It includes the following steps:

1. Dissolution: dissolve the crude heparin with water, keep warm and stir at a uniform speed;

2. Trypsin pretreatment: dissolve in deionized water, stir violently, heat up, add ammonia, and react

In the process, the pH value shall be controlled, the precipitate shall be washed with absolute ethanol, and finally dried under vacuum for standby.

3. Enzymolysis: add trypsin into the reaction solution, adjust the pH value, reach a certain temperature for enzymolysis and filtration to obtain

Filtrate, weigh the filtrate and collect trypsin.

4. Oxidation: add H2O2 to the filtrate in step 3 and continuously adjust the value. During oxidation, adjust the pH value;

5. Dissolution: add sodium chloride to step 4 and stir to dissolve it.

6. Ion exchange: the filtrate obtained in step 5 passes through the column with ion exchange resin, collects the effluent, adds lithium salt and stirs

dissolution.

7. Ultrafiltration to remove salt and water: concentrate with nanofiltration membrane and collect the concentrated solution.

8. The concentrated solution obtained in precipitation and drying step 7 is precipitated with ethanol, the above clear solution is sucked and washed repeatedly,

Until it becomes hard particles, then it is dried in vacuum and crushed by ball mill to obtain high-quality heparin lithium.

Desheng company is a company specializing in the production of blood collection reagents. At present, our heparin lithium has gone to the national market and received customers

Household recognition, including biological buffer, chemiluminescence reagent, enzyme preparation and other products are also gradually updated, which can provide you with

The best quality products and prices!