heparin lithium as anticoagulant

Details:

Sodium salt and lithium salt are common in clinical blood test, which has unique application value. Heparin has low chelating force, little impact on water molecule movement, little interference on blood components, no impact on red blood cell volume and no hemolysis. Therefore, heparin is recommended as an anticoagulant in a variety of tests based on whole blood or plasma. It is suitable for erythrocyte fragility test, blood gas analysis, hematocrit test, hemorheology and emergency biochemical determination. In the detection of pH value, blood gas, electrolyte and calcium ions, heparin is the only anticoagulant that can be used, and heparin lithium has the least possibility of interference in the detection of non lithium ions. Therefore, heparin lithium is recommended as an anticoagulant. At present, heparin lithium is gradually replacing heparin sodium in blood testing.

Heparin lithium is a chemical and an important member of blood anticoagulant. The appearance is white to almost white powder, and its CAS number is 9045-22-1. It is divided into 150u, 160u, 170u and 180u titers. The commonly used heparin anticoagulants include sodium, potassium, lithium and ammonium salts of heparin, of which heparin lithium is the first.

Application of heparin lithium anticoagulant:

1. For biochemical test of patients after hemodialysis: the blood sample of patients after hemodialysis is a special sample faced by the laboratory department of many hospitals. Many inspectors often feel helpless when facing such samples. This is because during hemodialysis, dialysis patients use anticoagulant drugs containing heparin, such as low molecular weight heparin calcium. At this time, due to a certain amount of heparin anticoagulant drugs in the blood, it is very difficult to coagulate and separate the bleeding serum after blood extraction in the common tube or gel / coagulant tube.

It is reported that there is bias in the analysis of blood K detected by heparin lithium anticoagulant plasma and serum, which may be caused by the high speed of blood sample during centrifugation, the extrusion damage and hemolysis of red blood cells in fibrin clots, and the release of K ions in red blood cells to serum. In addition, a small amount of red blood cells are inevitably destroyed during the transportation and coagulation of blood samples, resulting in a slight high level of blood K.

It was also reported that there were significant differences in the results of CK-MB, LDH and Glu between heparin lithium anticoagulant plasma and serum, which may be due to hemolysis or blood glucose decomposition after long storage time. On the contrary, heparin anticoagulant has the advantages of not affecting cell volume and not easy to cause hemolysis. It does not need to be placed in a water bath and can be centrifuged directly. The blood K, Glu and LDH measured by heparin lithium anticoagulant plasma are more accurate than the real concentration in the patient. Therefore, establishing the reference value system of plasma biochemical test as soon as possible will help clinicians make an accurate judgment on the patient's condition.

2. For routine biochemical test: the research results show that most routine biochemical test items can use heparin lithium anticoagulant plasma instead of serum, but the plasma reference range should be established for Glu, K +, Na +, CI -, P3 -, and the plasma test results can be explained by the serum reference range for other items; LP (a), PA, HBDH, LDH, CK, CKMB, IgM and other items should not be measured with heparin lithium anticoagulant plasma samples. Plasma Lp (a) increases with the increase of heparin concentration.

Desheng has 15 years of rich experience in the R & D and production of blood anticoagulants. It is an old manufacturer of blood collection additives. The purity of heparin lithium and heparin sodium produced is high, and the packaging can be formulated according to customer needs. Desheng biochemical insists on providing customers with high-quality products and professional services.