Lumbrokinase Powder

Details:

1. Introduction

Lumbrokinase（abbreviated as LK）are a group of serine proteases with fibrinolysis and/or plasminogen activation. Lumbrokinase powder is extracted from earthworms. It is widely present in different species of earthworms. It is rich in acidic amino acids, with a molecular weight of 20,000 to 40,000 Daltons, with dual functions of kinase and plasmin: It can not only directly degrade fibrin and fibrinogen like plasmin.

 

2. Main Functions

2.1. Thrombolytic effect

As a biological gene active enzyme product, its most representative function is the extremely strong thrombolytic effect. Its mechanism of action is: after LK enters the body, it quickly combines with fibrin, the main component of thromboembolism, and hydrolyzes it to achieve the purpose of dissolving the thrombus. Lumbrokinase powder not only hydrolyzes plasminogen-rich fibrin, but also hydrolyzes plasminogen-free fibrin particles. Therefore, it also has a powerful effect of thrombolysis and tongmai for new thrombosis and old thrombosis.

2.2. Anticoagulative effect

Through the blood tracking of LK, it was found that LK can adsorb coagulation factors and hydrolyze them, and at the same time inhibit the further adhesion of platelets on its surface. Therefore, LK regulates the existing thrombus while preventing the occurrence of new thrombus, which solves the medical problem that the wall thrombus often falls off and forms embolism in other tissues during the conditioning of thrombus, and enhances the safety of thrombolysis. . At the same time, LK has strong targeting in the process of thrombolysis and anticoagulation. It only adsorbs and hydrolyzes coagulation factors and platelets that have a tendency to coagulate, and does not destroy the blood coagulation mechanism, so thrombolysis and bleeding will not occur.

2.3. Activating enzyme efficacy

Clinical observation found: LK activates the fibrinolytic system in vivo. It forms a long-term stable internal environment that prevents thrombosis in the human body, and has a long-term antithrombotic effect. In addition, lumbrokinase can significantly prolong the occlusion time of blood vessels, so that the blood vessels do not need external drugs, but improve blood flow through their own elasticity regulation, complete tissue perfusion, and enhance tissue vitality.

 

2.4. The effect of activating cells

LK is the most active proteolytic enzyme, which can activate various cells in the human body, especially for cells in shock and semi-shock state in tissues with insufficient blood supply, which can quickly restore normal physiological functions and biological activities. Therefore, LK has an excellent promoting effect on the recovery of cerebral thrombosis, sequelae of cerebral infarction and myocardial infarction.

 

3. Applications

Lumbrokinases powder is mainly used in medicine to treat ischemic cerebrovascular diseases.

 

4. Flow Chart

Fresh earthworm washing→grinding→slagging→salting out→centrifugation→ultrafiltration→column chromatography separation→ultrafiltration→filtration→sterilization→drying→lumbrokinase

 

5. Quality Standard

According to Enterprise Standard

Items	Specifications	Results
Character	Light yellow powder, with hygroscopicity	Complies
Odor	Characteristic	Characteristic
Identification
1	Max absorption at 278nm	Complies
2	Positive reaction	Positive reaction
Heavy metals
Pb	≤1.5ppm	<1.5ppm
As	≤1ppm	<1ppm
Hg	≤0.1ppm	<0.1ppm
pH	6.0-8.0	7.3
Loss   on drying	≤6.0%	3.3%
Potency	Lumbrokinase unit activity/mg ≥16000	17716
Specific activity	≥20000	27592

 

6. Method of Analysis

MOA is available upon request

7. Reference Activity