Bovine Serum Albumin Powder

Details:

1. Introduction

Bovine Serum Albumin (BSA), also known as the fifth component, is a polypeptide chain composed of 583 amino acid residues, with 17 pairs of disulfide bonds in the peptide chain, a molecular weight of 66.43kD, and an isoelectric point of 4.7. Kono Chemical is specialized in the production of BSA freeze-dried powder, we do not use ammonium sulfate in the production, the product has high purity and small batch difference

2. Main Functions

2.1. BSA contributes to the stability and activity of Taq enzyme in the PCR system, and can improve the efficiency of PCR.

2.2 Plasmodium culture

In vitro continuous culture of Plasmodium is an important tool for the study of Plasmodium, however, fresh human serum necessary for the maintenance of Plasmodium growth is difficult to obtain. The researchers found that the addition of lipid-rich bovine serum albumin to the Plasmodium falciparum medium can effectively replace human serum, which is suitable for continuous in vitro culture of Plasmodium in the intra-erythrocyte stage. Or add human serum and lipid-rich bovine serum albumin into the Plasmodium medium in a certain proportion, and the Plasmodium can reach a growth state similar to that of whole human serum culture, thus reducing the complete dependence on fresh human serum.

2.3 Liver cancer research

High concentrations of free fatty acids (FFAs) and tumor necrosis factor (TNF-α) can alter cellular activity and cause lipotoxicity. The researchers found that lipid accumulation within cells reduces the lipotoxicity of free saturated fatty acids. Therefore, in order to develop effective treatments for diseases caused by complex and multiple influencing factors, and to search for genes that regulate multiple cellular processes, the researchers established a model in which human hepatoma cells (HepG2) were exposed to high concentrations of FFA and TNF-α in the physiological environment. The whole experiment was divided into control group, BSA group, palmitate group, oleate group and linoleate group, and then the accumulation of triglyceride (TG), toxicity and gene response in cells added with different FFA were detected.

3. Applications

 

3.1. For biochemical research, genetic engineering and medical research

3.2. Used as medicine and health food, condiment

3.3. Maintain osmotic pressure, pH buffering, carrier function

3.4. In the PCR system, it helps the stability and activity of Taq enzyme, which can improve the efficiency of PCR

            

4. Flow Chart

Fresh bovine blood→add hirudin→centrifugation→remove some impurities in blood→mix plasma with sodium chloride, potassium chloride, etc.→semipermeable membrane→obtain bovine serum albumin→mix the centrifugation residual liquid with the lower solution of the semipermeable membrane Mixing → adding low temperature absolute ethanol → stirring → precipitation of bovine serum albumin → drying →BSA

 

5. Quality Standard 

According to Enterprise Standard

Items	Specifications	Results
Appearance	Light beige powder	Light beige powder
Total protein	≥96.0%	96.38%
Albumin purity	≥98.0%	98.52%
A403 of 1% solution	≤0.1%	0.04%
Moisture	≤3.0%	1.25%
Heavy Metals	≤10ppm	<10ppm
pH	6.5～7.5（1% aqueous solution, 25℃）	7.1
Solubility	Dissolution time≤10 min (10% aqueous solution, 25℃)	Complies

Comparision between 3 different grades of BSA

 

6. Method of Analysis 

MOA is available upon request. 

 

7. Protease IgG test