China Biggest Manufacturer supply Highest Quality Pharm Grade 99.9% Guanidine Hydrochloride CAS 50-01-1

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Guanidine hydrochloride Basic information description Protein Denaturation Uses Drug intermediates Preparation Reference quality standards References Product Name: Guanidine hydrochloride Synonyms: guanidine,monohydrochloride;guanidinechloride;Guanidinemonohydrochloride;guanidiniumhydrochloride;AMINOFORMAMIDINE HCL;AMINOFORMAMIDINE HYDROCHLORIDE;AMINOMETHANAMIDINE HCL;AMINOMETHANAMIDINE HYDROCHLORIDE CAS: 50-01-1 MF: CH5N3.ClH MW: 95.53 EINECS: 200-002-3 Product Categories: Disinfectants;Test Reagents;Pharmaceutical Intermediates;Miscellaneous Biochemicals;Inhibitors;PP;proteinmod;50-01-1 Mol File: 50-01-1.mol   Guanidine hydrochloride Chemical Properties Melting point  180-185 °C(lit.) density  1.18 g/mL at 25 °C(lit.) refractive index  n20/D 1.465 storage temp.  room temp solubility  H2O: 6 M, clear, colorless form  Crystals color  White to slightly yellow Odor Odorless PH 4.5-5.5 (100g/l, H2O, 20℃) PH Range 4.5 - 6.0 at 573 g/l at 25 °C Water Solubility  2280 g/L (20 ºC) λmax λ: 260 nm Amax: 0.040 λ: 280 nm Amax: 0.025 Sensitive  Hygroscopic Merck  14,4562 BRN  3591990 Stability: Stable. Hygroscopic. Incompatible with strong oxidizing agents. InChIKey PJJJBBJSCAKJQF-UHFFFAOYSA-N CAS DataBase Reference 50-01-1(CAS DataBase Reference) EPA Substance Registry System Guanidine monohydrochloride (50-01-1)   Safety Information Hazard Codes  Xn,Xi Risk Statements  22-36/38 Safety Statements  26-36-22 WGK Germany  1 RTECS  MF4300000 F  3-10 TSCA  Yes HS Code  29252000 Toxicity LD50 orally in Rabbit: 655.3 - 907.1 mg/kg LD50 dermal Rabbit > 2000 mg/kg MSDS Information Provider Language Guanidine hydrochloride English ACROS English SigmaAldrich English ALFA English   Guanidine hydrochloride Usage And Synthesis description Guanidine hydrochloride is a strong organic base existing primarily as guanidium ions at physiological pH. It is found in the urine as a normal product of protein metabolism. It is also used in laboratory research as a protein denaturant.It is also used in the treatment of myasthenia and as a fluorescent probe in HPLC. Guanidine Hydrochloride is the hydrochloride salt form of guanidine, a strong basic compound with parasympathomimetic activity. Guanidine hydrochloride enhances the release of acetylcholine following a nerve impulse and potentiates acetylcholine actions on muscarinic and nicotinic receptors. It also appears to slow the rates of depolarization and repolarization of muscle cell membranes. Guanidine Hydrochloride is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids. Guanidine Hydrochloride is useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins and isolation of RNA. At higher concentrations, this strong denaturant has been reported to solubilize denatured insoluble proteins such as inclusion bodies. When utilized at lower concentrations, Guanidine Hydrochloride is noted to be useful in prompting the refolding of denatured proteins. Guanidine Hydrochloride is also been reported to allow return of enzymatic activity in protocols using Guanidine Hydrochloride as a first step. Guanidine Hydrochloride is an inhibitor of RNase. Guanidine hydrochloride is used for purification of proteins and nucleic acids. It can be used as a medicine, organic synthesis intermediate and is used in dyes. It acts as an intermediate in the preparation of sulfadiazine, which is an important raw material for sulfamethyldiazine, sulfamethazine and folic acid. It is utilized in the synthesis of 2-amino-pyrimidine, 2-amino-6-methyl-pyrimidine and 2-amino-4,6-dimethyl-pyrimidine. Also used in RNA isolation to dissociate nucleoproteins and inhibit RNase. Protein Denaturation Guanidine Hydrochloride (GdnHCl) is a better denaturant than urea.It should normally give higher and lower values for m and for Cm, respectively, but identical deltaG° values. Since m values in both denaturants can be predicted/estimated from the size of the protein (Myers et al., 1995) and delatG° should not change, yes it is possible-in principle-to predict Cm in one denaturant, knowing its value in the other. but to have a general correlation co-efficient is almost impossible as every protein has unique folding. Though such co-relation may be found for a special protein fold, but what purpose it may serve apart from giving a tentative idea that GdnHCl or urea may be a better denaturant! If you are looking for even better denaturant than Gdn HCl then use GdnSCN, it is better and more effective Uses Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase. Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Drug intermediates Guanidine hydrochloride drug used primarily as an intermediate in the manufacture of sulfadiazine, an important raw material sulfamethyldiazine, sulfamethazine and folic acid. Guanidine hydrochloride (or guanidine nitrate) and ethyl cyanoacetate reaction, cyclization of 2,4-diamino-6-hydroxy pyrimidine, folic acid for the synthesis of antianemic. Furthermore guanidine hydrochloride also used synthetic anti-static agent. Preparation In Dicyanodiamide and ammonium (Ammonium chloride) as raw material, melt reaction at 170-230 ℃, guanidine hydrochloride obtained crude, refined products. Reference quality standards Name of Project Specifications Pharmaceutical grade industrial grade Appearance white crystal; white crystal Content > 99% 99.5% Ammonium <0.3% 0.5% Water Insoluble substance <0.1% 0.1% Moisture <0.3% 0.3% Ash <0.05% 0.05% Water-soluble test qualified; qualified Melting range °C 184-188; 182-188 Absorbance (UV wavelength 260NM) <=0.04 Absorbance (UV wavelength 280NM) <=0.02 PH value (4% aqueous solution) 6.4 ± 0.2 6.4 ± 0.2 References # # # # Chemical Properties White cryst. powder Uses cholinergic Uses Guanidine has a good bacterial and fungicidal efficacy when used in low concentration and can also be used as disinfectant. Uses Used to synthesis medicine, pesticide, dye and other organic compound, is the important material to make sulfanilamide medicine and folacin, and can be used as anti-static agent of compound fibre. Uses Guanidine hydrochloride is used for purification of proteins and nucleic acids. It can be used as a medicine, organic synthesis intermediate and is used in dyes. It acts as an intermediate in the preparation of sulfadiazine, which is an important raw material for sulfamethyldiazine, sulfamethazine and folic acid. It is utilized in the synthesis of 2-amino-pyrimidine, 2- amino -6- methyl-pyrimidine and 2-amino -4,6 - dimethyl-pyrimidine. Also used in RNA isolation to dissociate nucleoproteins and inhibit RNase. Definition ChEBI: An aminocarboxamidine, the parent compound of the guanidines. Application Guanidine hydrochloride can be used as pharmaceuticals, pesticides, dyes and other organic synthesis intermediates. It can be used to synthesize 2-aminopyrimidine, 2-amino-6-methylpyrimidine, 2-amino-4,6-dimethylpyrimidine, and is used for the manufacture of sulfadiazine, sulfamethazine, sulfamethazine and other sulfa drugs. Intermediate. Guanidine hydrochloride (or guanidine nitrate) reacts with ethyl cyanoacetate to form 2,4-diamino-6-hydroxypyrimidine, which is used to synthesize the anti-anemia drug folic acid. It can also be used as an antistatic agent for synthetic fibers. Can also be used for protein denaturants. As a strong denaturant in experiments for extracting total cellular RNA[3]. The guanidine hydrochloride solution can dissolve the protein, leading to the destruction of the cell structure, the destruction of the secondary structure of the nucleoprotein, and the dissociation from the nucleic acid. In addition, the RNase can be inactivated by reducing agents such as guanidine hydrochloride. General Description Guanidine hydrochloride (GdnHCl) is a well-known protein and enzyme denaturant. It is a small molecule and hydroscopic in nature. Hazard Toxic by ingestion, evolves hydrogen chloride fumes on heating. Biochem/physiol Actions Guanidine hydrochloride (GdnHCl) is involved in conformational changes and loss of enzyme activity due to inactivation of the enzyme. It is involved in the loss of activity of alkaline phosphatase (ALPase) enzyme in Haliotis diversicolor. GdnHCl functions by hampering the activity of heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) in vivo. It can also inactivate enzymes like papain and aminocyclase. Higher concentrations of GdnHCl leads to viral inactivation of herpes simplex virus 1 (HSV-1). Purification Methods Crystallise the hydrochloride from hot methanol by chilling to about -10o, with vigorous stirring. The fine crystals are filtered through fritted glass, washed with cold (-10o) methanol, dried at 50o under vacuum for 5hours. (The product is purer than that obtained by crystallisation at room temperature from methanol by adding large amounts of diethyl ether.) [Kolthoff et al. J Am Chem Soc 79 5102 1957, Beilstein 3 H 86, 3 II 71, 3 III 160, 3 IV 150.]   Guanidine hydrochloride Preparation Products And Raw materials Raw materials Ammonium chloride Preparation Products 2-Aminopyrimidine-->5-Amino-2-chloropyrimidine-->2,5-Diamino-4,6-dichloropyrimidine-->7-(TRIFLUOROMETHYL)-3-IODOIMIDAZO[1,2-A]PYRIMIDINE-->3-Bromo-7-(trifluoromethyl)imidazo[1,2-a]pyrimidine-->5-AMINO-2-FLUOROPYRIMIDINE-->2-Chloro-5-nitropyrimidine-->9-ETHYLGUANINE-->Guanidine carbonate-->FORMAMIDINE HYDROCHLORIDE-->2-Amino-5-pyrimidinecarboxyaldehyde-->ISOXANTHOPTERIN-->2-AMINO-PYRIMIDINE-5-CARBOXYLIC ACID ETHYL ESTER-->2-Aminopyrimidine-5-carboxylic acid-->2-AMINO-6-HYDROXY-PYRIMIDINE-4-CARBOXYLIC ACID-->2-Amino-5-nitropyrimidine-->2-Amino-4-chloro-6-methylpyrimidine-->2-amino-6-chloropyrimidin-4(3H)-one-->GUANINE SULFATE-->2,4-DIAMINO-1,3,5-TRIAZINE-->2-Amino-6-methyl-4-pyrimidinol-->Amiloride hydrochloride-->2-Amino-6-hydroxypyrimidin-4(3H)-one ,97%-->2,4-Diaminopyrimidine-->shark soft bone preparation-->6-HYDROXY-2,4,5-TRIAMINOPYRIMIDINE-->Guanidine phosphate Tag:Guanidine hydrochloride(50-01-1) Related Product Information

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Leader Biochemical Group is a large leader incorporated industry manufacturers and suppliers of advanced refined raw materials From the year of 1996 when our factory was put into production to year of 2020, our group has successively invested in more than 52 factories with shares and subordinates.We focus on manufacture Pharm & chemicals, functional active ingredients, nutritional Ingredients, health care products, cosmetics, pharmaceutical and refined feed, oil, natural plant ingredients industries to provide top quality of GMP standards products.All the invested factories' product lines cover API and intermediates, vitamins, amino acids, plant extracts, daily chemical products, cosmetics raw materials, nutrition and health care products, food additives, feed additives, essential oil products, fine chemical products and agricultural chemical raw materials And flavors and fragrances. Especially in the field of vitamins, amino acids, pharmaceutical raw materials and cosmetic raw materials, we have more than 20 years of production and sales experience. All products meet the requirements of high international export standards and have been recognized by customers all over the world. Our manufacture basement & R&D center located in National Aerospace Economic & Technical Development Zone Xi`an Shaanxi China. Now not only relying on self-cultivation and development as well as maintains good cooperative relations with many famous research institutes and universities in China. Now, we have closely cooperation with Shanghai Institute of Organic Chemistry of Chinese Academy of Science, Beijing Institute of Material Medical of Chinese Academy of Medical Science, China Pharmaceutical University, Zhejiang University. Closely cooperation with them not only integrating Science and technology resources, but also increasing the R&D speed and improving our R&D power. Offering Powerful Tech supporting Platform for group development. Keep serve the manufacture and the market as the R&D central task, focus on the technical research.  Now there are 3 technology R & D platforms including biological extract, microorganism fermentation and chemical synthesis, and can independently research and develop kinds of difficult APIs and pharmaceutical intermediates. With the strong support of China State Institute of Pharmaceutical Industry (hereinafter short for CSIPI), earlier known as Shanghai Institute of Pharmaceutical Industry (SIPI), we have unique advantages in the R & D and industrialization of high-grade, precision and advanced products.  Now our Group technical force is abundant, existing staff more that 1000 people, senior professional and technical staff accounted for more than 50% of the total number of employees, including 15 PhD research and development personnel, 5 master′ S degree in technical and management personnel 9 people. We have advanced equipment like fermentation equipment and technology also extraction, isolation, purification, synthesis with rich production experience and strict quality control system, According to the GMP required, quickly transforming the R&D results to industrial production in time, it is our advantages and our products are exported to North and South America, Europe, Middle East, Africa, and other five continents and scale the forefront in the nation, won good international reputation.  We believe only good quality can bring good cooperation, quality is our key spirit during our production, we are warmly welcome clients and partner from all over the world contact us for everlasting cooperation, Leader will be your strong, sincere and reliable partner in China.

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