D-Lysine homopolymer hydrobromide

Details:

D-Lysine homopolymer hydrobromide Basic information
Product Name:    D-Lysine homopolymer hydrobromide
Synonyms:    POLY-D-LYSINE HYDROBROMIDE MOL WT*15;POLY-D-LYSINE HYDROBROMIDE MOL WT*70;POLY-D-LYSINE HYDROBROMIDE MOL WT*GR;POLY-D-LYSINE HYDROBROMIDE*MOL WT 30;POLY-D-LYSINE HYDROBROMIDE, MOL WT&;Poly-D-lysine hydrobromide, M.W. 30,000 to 70,000;D-LYS-(D-LYS)N-D-LYS XHBR;POLY-D-LYSINE 70 000 HBR
CAS:    27964-99-4
MF:    C6H14N2O2
MW:    146.19
Mol File:    27964-99-4.mol

D-Lysine homopolymer hydrobromide Chemical Properties
storage temp.     -20°C
form     lyophilized powder
Water Solubility     Soluble in water.

D-Lysine homopolymer hydrobromide Usage And Synthesis
Uses    Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.
Uses    Poly-D-lysine hydrobromide is used for the following applications:
Used in animal cell culture
Rat cortical neuron cultures
Cell culture and siRNA treatment
Primary cell cultures and adult neuronal network model
Cortical astrocyte culture
Immunofluorescence staining (HDF cells were plated onto poly-D lysine (P0899, Sigma-Aldrich) coated glass coverslips)
NPC collection and culture conditions
Biochem/physiol Actions    This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.