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77-86-1 Trometamol Tromethamine
CAS:
77-86-1
MF:
C4H11NO3
MW:
121.14
EINECS:
201-064-4
MDL No.:
MFCD00004679
Melting point:
167-172 °C(lit.)
Boiling point:
219-220 °C10 mm Hg(lit.)
Density
1,353 g/cm3
vapor pressure
0.0267 hPa (20 °C)
refractive index
1.4170 (estimate)
Flash point:
219-220°C/10mm
storage temp.
20-25°C
solubility
H2O: 4 M at 20 °C, clear, colorless
form
crystalline
color
white
Application:Tris base, also known as THAM, is widely used as a biological buffer or as a component of buffer formulations such as TAE and TBE buffers. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.
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