UBIQUITIN CAS 79586-22-4

Details:

ndard:BP/USP/EP/Enterprise standard

2) All Purity≥99%

3) We are manufacturer and can provide high quality products with factory price.

 

2, Fast and safe delivery

1) Parcel can be sent out in 24 hours after payment.Tracking number available

2) Secure and discreet shipment.Various transportation methods for your choice.

3) Customs pass rate ≥99%

4) We have our own agent/remailer/distributor who can help us ship our products very fast and safe,

and we have stock in there for transferring.Our Service:
1. Fast Delivery: We can delivery within 24 hours upon receipt of your payment.
2. Quality can be promised. Hot sell to Worldwide.
3. Payment Terms: T/T,, t/t
4. Free Sample available at any time.
5. Tracking your order at any time. Inform your order's further new situation at any time.

Product Description:

The Recombinant SUMO Protease (Small ubiquitin-related Modifier Protease) is a commonly used UBL-specific Protease (UBL-specific Protease 1, Ubiquitin-like protein-specific protease 1 (ULP1) can recognize the tertiary structure of SUMO (Small ubiquitin-like Modifier) protein with high specificity. Hydrolyze the post-Gly-Gly peptide bond in the X-Gly-Gly-X (Except Pro) peptide at the carboxy-terminal of SUMO protein, so that the target protein released after cleavage of the fusion protein by this protease does not contain excess amino acids.The optimum pH and temperature for Sumo Protease digestion were 8.0 and 30ºC.It has good stability and high enzyme activity in pH range 6.0-10.0, temperature range 2-30ºC, ionic strength range 0-400 mM NaCl.In practice, in order to preserve the structure and biological activity of the target protein as much as possible, it is recommended to use Recombinant Sumo Protease digestion at 4ºCovernight, and at the same time, adding appropriate concentration of DTT (0.5-2 mM) into the digestion system can significantly improve the digestion efficiency.

Application: It is commonly used to remove the SUMO tagged protein from fusion proteins.The protease itself carries the HIS label and binds to the nickel column.

Source: Saccharomyces cerevisiae cysteine protease, recombinant expression of Escherichia coli, with HIS label.

Enzyme activity definition: one unit of enzyme activity can be used to cut more than 85% of 2 μg of substrate (containing SUMO labeled protein) within 1 h at 30ºC, pH8.0.

Inactivation or inhibition: Imidazole higher than 150 mM will affect the digestion efficiency of Recombinant SUMO Protease

Purity and concentration: SDS-PAGE test purity ≥95%, 10 U/μL.

Recombinant SUMO protease storage solution:25 mM Tris-HCl,pH 8.0,0.1% NP-40,250 mM NaCl,500 μM DTT,50% (v/v) glycerol.

10×SUMO Buffer+Salt:500 mM Tris-HCl,pH 8.0,2% NP-40,1.5 M NaCl,10 mM DTT

10×SUMO Buffer - Salt:500 mM Tris-HCl,pH 8.0,2% NP-40,10 mM DTT.

   

Component Number	Component	G3411-2000U
G3411-1	Recombinant SUMO Protease (His-tag) (10 U/μL)	200 μL
G3411-2	10×SUMO Buffer + Salt	1 mL
G3411-3	10×SUMO Buffer - Salt	1 mL
G3411-4	Control Protein	100 μL

 

Product Usage:

1.Portenase digestion system recommendation

Component	Volume
10×SUMO Buffer +/- Salt	5 μL
Interest protein	20 μg
Recombinant TEV Protease (His-tag) (10 U/μL)	1 μL
ddH2O	To 50 μL

2. The reaction system was placed at 4ºC for overnight reaction or 16h;

3. 10μL sample of digestion product was taken for SDS-PAGE detection to evaluate the amount of digestion and reaction conditions.

 

Our company specializes in processing and selling chemical raw materials, chemical products, pharmaceutical intermediates, veterinary medicine intermediates, dye intermediates, pigments, cosmetics raw materials and chemical reagents,  

Our company has a number of sales team, service and information feedback vertical integration of a sound marketing network, products are sold throughout the country and exported to South Asia, Europe, America, Africa and other more than 20 countries and regions.  

Companies adhering to the "pursuit of quality, innovation and development" business philosophy, to serve as a foundation, for the survival by the quality, seek development by science and technology, adhere to quilty comes first,fair price,good sense of customer service and responsibility. in the future hebei Nengqian import and export trade Co.,Ltd will always hold "integrity, innovation" business philosophy, to achieve honesty management, on the way of specialization, internationalization,  Keep moving!  

Packing:  
  1. Inner double plastic bags----25kg/Fiber drum (35*35*45cm, GW: 28kg, NW: 25kg); 

  2. Inner double plastic bags----5kg/Aluminum foil bag (GW: 6.5kg, NW: 5kg). 

  3. Inner double plastic bags----1kg/Aluminum foil bag (GW: 1.5kg, NW: 1kg). 

P.S.: we accept packaging customization. 
  
Delivery:   

  1. Stock products are normally shipping within 3 working days.  

  2. For rush delivery, please contact our sales team for particular arrangement. 

Shipping Details: 
  1. We ship goods via DHL, Fedex, UPS, TNT, China post, NL Post and other couriers, weight from 10g to 1000kg or even bulker. 

  2. Shipping details & shipping documents will be provided and sent by email. 

  3. We keep tracking the shippment until clients well received. 

  4. After years export shipping experience, with professional and experienced cooperated forwarders, we ensure that goods can be delivered in multiple ways safetly and efficiently. 

FAQ: 
1. Are you a trade company or factory   
We are a  factory with our own trading company.  

2. How do you control the quality   
   Our factory is equipped with professional technicians to control quality, out inspectors take sample for testing every 2 hours to ensure the quality of our production. We also accept BV, SGS or any other Third-party inspection.   
3.  How long is lead time   
   We deliver goods within 3 days for small order, 7-10 days for bulk order.   
4.  Where is your factory located  How can I visit the factory   
    Our factory located in WEIFANG, China. It is only two hours from QINGDAO.  

6. Can i get some samples  
  Yes, we can supply free sample, but the shipping cost be paid by our customers. 

7. How to start orders or make payments  
  Proforma invoice will be sent first after confirmation of order, enclosed our bank information.Payment by T/T,  

8. How to confirm the Product Quality before placing orders  
  You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. 
  You can send us your product specifications and requests,we will manufacture the products according to your requests. 

9. How long is lead time

We deliver goods within 3 days for small order, 7-10 days for bulk order.

 

10. How do you treat quality complaint

  First of all, our quality control will reduce the quality problem to near zero. If there is a realquality problem caused by us, we will send you free goods for replacement or refund your loss.