Agarose CAS9012-36-6

Details:

Density 1.8± 0.1g /cm3
Boiling point 993.9±65.0 °C at 760 mmHg
Melting point ≤90 °C (4% in water)(lit.)
Molecular formula C24H38O19
Molecular weight: 630.547
Flash point 554.9±34.3 °C
Accurate mass 630.200745
PSA 285.37000
LogP minus 2.85
Appearance suspension (75% in water) | White to slightly cream
Vapor pressure 0.0±0.6 mmHg at 25°C
Refractive index 1.679
Storage condition
Room temperature closed light, ventilated dry place

stability
Analysis Note The following is a list of properties associated with our agaroses:

Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.

Gel strength - the force that must be applied to a gel to cause it to fracture.

Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit  hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.

Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the  matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix,  giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode,  EEO can disrupt separations because of internal convection.

Application Routine use agarose is ideal for everyday analysis of nucleic acids by gel electrophoresis or blotting  (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion  (RID). Has low ethidium bromide and SYBR Green background staining.

Water solubility H2O: 20 mg/mL hot, slightly hazy, very faintly yellow | Soluble in water.
Computational chemistry
1. Reference value for calculating hydrophobic parameters (XlogP) : None

2. Number of hydrogen bond donors :0

3. Number of hydrogen bond receptors :1

4. Number of rotatable bonds :6

5. Number of tautomers :7

6. Topological molecular polar surface area 17.1

7. Number of heavy atoms :15

8. Surface charge :0

9. Complexity :178

10. Number of isotope atoms :0

11. Determine the number of primary constitutive centers :0

12. Number of indeterminate atomic orthotropic centers :0

13. Determine the number of chemical bond constitutive centers :0

14. Number of uncertain bond constitutive centers :0

15. Number of covalent bond units :1

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