High Quality Fluorescein isothiocyanate isomer I / FITC Powder CAS 3326-32-7 For Biochemical Studies / Scientific Research

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Description of Fluorescein isothiocyanate isomer I from Wuhan Fortuna Chemical Co.,Ltd

Basic Information of Fluorescein isothiocyanate isomer I
Product Name	Fluorescein isothiocyanate isomer I
CAS NO.	3326-32-7
Apperance	Yellow powder
Assay	98.42%
Pacakge	1kg/Foil Bag,25kg/Drum/Box
MOQ	1kg
Storage	Cool and dry place
Shelf Life	2 Years
Brand	Fortunachem

Specifications(COA) of High Quality Fluorescein isothiocyanate isomer I / FITC Powder CAS 3326-32-7 For Biochemical Studies / Scientific Research

Items	Specifications	Results
Appearance	Yellow powder	Yellow powder
Absorption Maximum	494±5nm	494nm
Extinction Coefficient(cm-1M-1)	65000±5000	68300
HPLC Purity	≧95%@254nm	98.42%
Storage Condition	0℃ desiccated,avoid light	0℃desiccated,avoid light
Shipping Condion	Room temperature	Room temperature
Conclusion	QUALIFIED.

 

Introduction of Fluorescein isothiocyanate isomer I.

Fluorescein isothiocyanate (FITC) isomer I is a fluorescent dye commonly used in various biological and biochemical applications. FITC is a derivative of fluorescein, a synthetic organic compound that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. The isothiocyanate group in FITC allows it to covalently bind to amine groups on proteins, antibodies, and other biomolecules, making it a versatile tool for labeling and detection.

Key Applications of FITC Isomer I:

Flow Cytometry:FITC is widely used in flow cytometry to label antibodies that bind to specific cell surface markers. This allows for the identification and quantification of different cell populations based on their fluorescence characteristics.

Fluorescence Microscopy:FITC-labeled antibodies or other probes can be used to visualize the localization of specific proteins or other molecules within cells and tissues under a fluorescence microscope.

Immunofluorescence:In immunofluorescence assays, FITC-conjugated antibodies are used to detect the presence and distribution of antigens in biological samples. This technique is commonly used in research and diagnostic laboratories.

Western Blotting:FITC can be used to label secondary antibodies in Western blotting, allowing for the detection of specific proteins on a membrane using fluorescence imaging systems.

ELISA (Enzyme-Linked Immunosorbent Assay):FITC-labeled antibodies can be used in ELISA to detect the presence of specific antigens in a sample, with the fluorescence signal providing a quantitative readout.

Live Cell Imaging:FITC can be used to label live cells for various imaging applications, including tracking cell movement, proliferation, and interactions in real-time.

Properties of FITC Isomer I:

Excitation and Emission: FITC has an excitation peak around 495 nm and an emission peak around 519 nm, which corresponds to its bright green fluorescence.

Reactivity: The isothiocyanate group (-N=C=S) in FITC reacts with primary amines (-NH2) on proteins and other biomolecules, forming a stable thiourea bond.

Solubility: FITC is generally soluble in organic solvents like dimethyl sulfoxide (DMSO) and dimethylformamide (DMF), and it can also be dissolved in aqueous buffers for biological applications.

Handling and Storage:

Light Sensitivity: FITC is light-sensitive and should be protected from prolonged exposure to light to prevent photobleaching.

Storage: FITC and its conjugates should be stored at low temperatures (e.g., -20°C) and in the dark to maintain stability and fluorescence intensity.

Overall, FITC isomer I is a powerful and widely used tool in molecular biology, immunology, and cell biology for the fluorescent labeling and detection of various biomolecules.

FAQ From Wuhan Fortuna Chemical Co.,Ltd
Q	Can I get a sample for free
A	For small QTY and low value product, we can provide sample for free exclude shipping cost. For high value sample, we will charge the basic sample cost, but we can refund all the cost if customers place order after sample test.
Q	How to confirm the product quality before placing orders
A	Most of our products confirm to international standards like USP/EP/BP/JP/CP. We will provide COA/TDS/MSDS for customers to confirm the specification before placing order.
Q	What is the payment you accept
A	For small and low value order, we accept CreditCard, MaterCard,VISA, and T/T.For large order, we can do T/T and L/C. Any pay on delivery or pay-after are not available for us.
Q	What is your shipment way
A	For less than 100kg gross weight order will ship with courier like FedEx or UPS, we support customers to place courier orders remotely by their account, we will package the goods well and wait for pick up.For more than 100kg goods, we will transport by Air or Sea according to customers' requirement.
Q	How long is your after-sales service
A	We accept customer’s quality complaint in three month after customer received the goods. We provide tech.support for all-life if customer place order from our company.
Q	How do you treat quality complaint
A	We support customer to provide our products to any rightful third party testing organization to test our products according to the COA specification, if the test result is confirm with COA, we will take all the loss for customers.

Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical and Intermediates, Food and Feed additives, Fine chemicals,Biochemical and Reagent, which is  the members of Hubei E-commerce Chamber.

 

Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients' requirement.

 

We have a positive and self-motivated management work team with a common philosophy, caring and commitment through teamwork, our team strive to achieve success in delighting our clients and ourselves.

 

Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward power, their satisfaction is our struggling goal.

 

Wuhan Fortuna Chemical Co.,Ltd is a professional manufacturer which provide Fluorescein isothiocyanate isomer I with good quality and best price, welcome to contact us to get COA/TDS/MSDS of Fluorescein isothiocyanate isomer I.