- Min.Order :10 Milligram
- Purity: ≥98%
- Payment Terms : T/T
Keywords
Anemarsaponin B 139051-27-7 standard supplier in China
Quick Details
- Appearance:detailed see specifications
- Application:analysis,activity test,Botanical Reference Materials,Standard materials
- PackAge:According to the clients requirement.
- ProductionCapacity:1|Metric Ton|Day
- Storage:Store at 2~8°C
- Transportation:by air or by ocean shipping
Superiority:
Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers.
With excellent quality products and good service, we have clients from more than dozens countries and regions, and we pride ourselves in providing our customers with a total satisfaction experiences.
We are doing our best to be your reliable partner for high quality Phytochemicals and Reference Standards from china.
Our main services:
A. Supply active ingredients and reference standards ofTraditional Chinese Medicine, from mgs to kgs scale.
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR, CM and PD services from lab scale, pilot scale to commercial scale(GMP is also available)
E.Traditional Chinese Medicine compounds library
1.Provide traditional Chinese medicine reference materials and natural active ingredients;
2.More than 2200 compounds are available for selection, continuously building high-quality natural product libraries for drug research and development;
3.Provide various screening libraries and more inhibitor products;
4.Provide separation and structural determination of natural products;
5.Laboratory scale pilot to commercial scale collaborative research and process development services.More than 180 experiences in phytochemistry (still increasing)
Each product has passed very strict testing (NMR/MS/HPLC)
Agents from many countries
General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging：1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition：Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.
Details:
Chemical Properties of Anemarsaponin B
| Cas No. | 139051-27-7 | SDF | |
| PubChem ID | 132198 | Appearance | Powder |
| Formula | C45H74O18 | M.Wt | 903.04 |
| Type of Compound | Steroids | Storage | Desiccate at -20°C |
| Solubility | Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc. | ||
| SMILES | CC1=C(OC2C1C3(CCC4C(C3C2)CCC5C4(CCC(C5)OC6C(C(C(C(O6)CO)O)O)OC7C(C(C(C(O7)CO)O)O)O)C)C)CCC(C)COC8C(C(C(C(O8)CO)O)O)O | ||
| Standard InChIKey | ROHLIYKWVMBBFX-DWGYQJTISA-N | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
||
| About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
||
| Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. | ||
Source of Anemarsaponin B
The rhizome of Anemarrhena asphodeloides Bunge.
Biological Activity of Anemarsaponin B
| Description | Anemarsaponin B can inhibit PAF-induced rabbit platelet aggregation in vitro. Anemarsaponin B has anti-inflammatory effect in LPS-treated RAW 264.7macrophages, the effect is associated with the inhibition of NF-κB transcriptional activity, possibly via the p38 MAP kinase pathway. |
| Targets | NOS | COX | p38MAPK | IL Receptor | TNF-α | NF-kB |
| In vitro |
[Studies on the active constituents of Anemarrhena asphodeloides bunge].[Pubmed: 1529709] Yao Xue Xue Bao. 1992;27(1):26-32.
A new active steroidal saponin from Anemarrhena asphodeloides.[Pubmed: 1798801] Planta Med. 1991 Oct;57(5):460-2.
|
Protocol of Anemarsaponin B
| Kinase Assay |
Anti-inflammatory effect of anemarsaponin B isolated from the rhizomes of Anemarrhena asphodeloides in LPS-induced RAW 264.7 macrophages is mediated by negative regulation of the nuclear factor-kappaB and p38 pathways.[Pubmed: 19375480] Food Chem Toxicol. 2009 Jul;47(7):1610-7. Anemarrhena asphodeloides is widely used in traditional Chinese medicine, and is known to have anti-diabetic and diuretic effects. |
Preparing Stock Solutions of Anemarsaponin B
| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 1.1074 mL | 5.5369 mL | 11.0737 mL | 22.1474 mL | 27.6843 mL |
| 5 mM | 0.2215 mL | 1.1074 mL | 2.2147 mL | 4.4295 mL | 5.5369 mL |
| 10 mM | 0.1107 mL | 0.5537 mL | 1.1074 mL | 2.2147 mL | 2.7684 mL |
| 50 mM | 0.0221 mL | 0.1107 mL | 0.2215 mL | 0.4429 mL | 0.5537 mL |
| 100 mM | 0.0111 mL | 0.0554 mL | 0.1107 mL | 0.2215 mL | 0.2768 mL |
| * Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. | |||||
References on Anemarsaponin B
[Studies on the active constituents of Anemarrhena asphodeloides bunge].[Pubmed:1529709]
Yao Xue Xue Bao. 1992;27(1):26-32.
From the ethanol extract of the Anemarrhena asphodeloides Buge (Liliaceae), a new steroidal suponin Anemarsaponin B and two known saponins anemarsaponin A1 and A2 were isolated. On the basis of chemical evidences and spectral analysis (UV, IR, EI-MS, FAB-MS, 1H-NMR and 13C-NMR), the structure of Anemarsaponin B was elucidated as 26-O-beta-D-glucopyranosylfurost-20(22)-ene-3 beta,26-diol-3-O-beta-D- glucopyranosyl(1----2)-beta-D-galactopyranoside. Preliminary pharmacological tests showed that Anemarsaponin B could inhibit PAF-induced rabbit platelet aggregation in vitro.
Anti-inflammatory effect of anemarsaponin B isolated from the rhizomes of Anemarrhena asphodeloides in LPS-induced RAW 264.7 macrophages is mediated by negative regulation of the nuclear factor-kappaB and p38 pathways.[Pubmed:19375480]
Food Chem Toxicol. 2009 Jul;47(7):1610-7.
Anemarrhena asphodeloides is widely used in traditional Chinese medicine, and is known to have anti-diabetic and diuretic effects. In this study, we evaluated the anti-inflammatory effects of Anemarsaponin B (ASB), a steroidal saponin isolated from the rhizomes of A. asphodeloides (Liliaceae), in LPS-stimulated RAW 264.7 macrophage cell line. ASB significantly and dose-dependently decreased the protein and mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). ASB also reduced the expressions and productions of pro-inflammatory cytokines, including those of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6). Electrophoretic mobility shift assay (EMSA) and reporter gene assays revealed that ASB attenuated the LPS-induced DNA binding and transcriptional activity of nuclear factor-kappa B (NF-kappaB). In addition, it was found that pretreatment with ASB significantly inhibited the nuclear translocation of the p65 subunit of NF-kappaB by blocking the phosphorylation of inhibitory kappa B-alpha (IkappaB-alpha). On the other hand, ASB inhibited the phosphorylation of MAP kinase kinases 3/6 (MKK3/6) and mixed lineage kinase 3 (MLK3), which are both involved in the p38 pathway. Taken together, these results suggest that anti-inflammatory effect of ASB in LPS-treated RAW 264.7 macrophages is associated with the inhibition of NF-kappaB transcriptional activity, possibly via the p38 MAP kinase pathway.
A new active steroidal saponin from Anemarrhena asphodeloides.[Pubmed:1798801]
Planta Med. 1991 Oct;57(5):460-2.
A new active steroidal saponin, Anemarsaponin B, was isolated from the rhizomes of Anemarrhina asphodeloides. The structure of Anemarsaponin B was elucidated as 26-O-beta-D-glucopyranosylfurost-20(22)-ene-3 beta, 26-diol-3-O-beta-D-glucopyranosyl-(1----2)-beta-D-galactopyranoside by chemical and spectral studies. Preliminary pharmacological tests showed that Anemarsaponin B could inhibit PAF-induced rabbit platelet aggregation in vitro.
Description
Anemarsaponin B is a steroidal saponin isolated from the rhizomes of A. asphodeloides (Liliaceae). Anemarsaponin B decreases the protein and mRNA levels of iNOS and COX-2. Anemarsaponin B reduces the expressions and productions of pro-inflammatory cytokines, including TNF-a and IL-6. Anemarsaponin B inhibits the nuclear translocation of the p65 subunit of NF-κB by blocking the phosphorylation of IκBα. Anemarsaponin B also inhibits the phosphorylation of MAP kinase kinases 3/6 (MKK3/6) and mixed lineage kinase 3 (MLK3). Anti-inflammatory effect .
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