- Min.Order :10 Milligram
- Purity: ≥98%
- Payment Terms : T/T
Keywords
Vanillin 121-33-5 standard supplier in China
Quick Details
- Appearance:detailed see specifications
- Application:analysis,activity test,Botanical Reference Materials,Standard materials
- PackAge:According to the clients requirement.
- ProductionCapacity:1|Metric Ton|Day
- Storage:Store at 2~8°C
- Transportation:by air or by ocean shipping
Superiority:
Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers.
With excellent quality products and good service, we have clients from more than dozens countries and regions, and we pride ourselves in providing our customers with a total satisfaction experiences.
We are doing our best to be your reliable partner for high quality Phytochemicals and Reference Standards from china.
Our main services:
A. Supply active ingredients and reference standards ofTraditional Chinese Medicine, from mgs to kgs scale.
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR, CM and PD services from lab scale, pilot scale to commercial scale(GMP is also available)
E.Traditional Chinese Medicine compounds library
1.Provide traditional Chinese medicine reference materials and natural active ingredients;
2.More than 2200 compounds are available for selection, continuously building high-quality natural product libraries for drug research and development;
3.Provide various screening libraries and more inhibitor products;
4.Provide separation and structural determination of natural products;
5.Laboratory scale pilot to commercial scale collaborative research and process development services.More than 180 experiences in phytochemistry (still increasing)
Each product has passed very strict testing (NMR/MS/HPLC)
Agents from many countries
General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging：1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition：Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.
Details:
Chemical Properties of Vanillin
| Cas No. | 121-33-5 | ||
| PubChem ID | 1183 | Appearance | White powder |
| Formula | C8H8O3 | M.Wt | 152.14 |
| Type of Compound | Phenols | Storage | Desiccate at -20°C |
| Synonyms | 4-Hydroxy 3-methoxybenzaldehyde; Vanillic aldehyde | ||
| Solubility | DMSO : ≥ 100 mg/mL (657.25 mM) *"≥" means soluble, but saturation unknown. |
||
| Chemical Name | 4-hydroxy-3-methoxybenzaldehyde | ||
| SMILES | COC1=C(C=CC(=C1)C=O)O | ||
| Standard InChIKey | MWOOGOJBHIARFG-UHFFFAOYSA-N | ||
| Standard InChI | InChI=1S/C8H8O3/c1-11-8-4-6(5-9)2-3-7(8)10/h2-5,10H,1H3 | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
||
| About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
||
| Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. | ||
Source of Vanillin
The roots of Beta vulgaris
Biological Activity of Vanillin
| Description | Vanillin is a single molecule extracted from vanilla beans and also a popular odor used widely in perfume, food and medicine.Vanillin can reversibly and non-competitively inhibit the cellulase activity at appropriate concentrations and the value of IC50 was estimated to be 30 g/L.Vanillin protects KSC from UVB irradiation and its effects may occur through the suppression of downstream step of MDM2 in UVB irradiation-induced p53 activation. Vanillin also inhibits yeast growth and fermentation. |
| Targets | Chk | p53 | p38MAPK | JNK | Mdm2 | NADPH-oxidase |
| In vitro |
Vanillin protects human keratinocyte stem cells against ultraviolet B irradiation.[Pubmed: 24184596] Food Chem Toxicol. 2014 Jan;63:30-7. Ultraviolet-B (UVB) irradiation is one of major factors which induce cellular damages in the epidermis. Importance of glucose-6-phosphate dehydrogenase (G6PDH) for vanillin tolerance in Saccharomyces cerevisiae.[Pubmed: 24725964] J Biosci Bioeng. 2014 Sep;118(3):263-9. Vanillin is derived from lignocellulosic biomass and, as one of the major biomass conversion inhibitors, inhibits yeast growth and fermentation. Vanillin was recently shown to induce the mitochondrial fragmentation and formation of mRNP granules such as processing bodies and stress granules in Saccharomyces cerevisiae. Furfural, another major biomass conversion inhibitor, also induces oxidative stress and is reduced in an NAD(P)H-dependent manner to its less toxic alcohol derivative. Therefore, the pentose phosphate pathway (PPP), through which most NADPH is generated, plays a role in tolerance to furfural. Although Vanillin also induces oxidative stress and is reduced to vanillyl alcohol in a NADPH-dependent manner, the relationship between Vanillin and PPP has not yet been investigated. |
Protocol of Vanillin
| Kinase Assay |
Vanillin causes the activation of Yap1 and mitochondrial fragmentation in Saccharomyces cerevisiae.[Pubmed: 23850265] Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass.[Pubmed: 24997375] Bioresour Technol. 2014 Sep;167:324-30. Pretreatment of lignocellulosic material produces a wide variety of inhibitory compounds, which strongly inhibit the following enzymatic hydrolysis of cellulosic biomass. Vanillin is a kind of phenolics derived from degradation of lignin. J Biosci Bioeng. 2014 Jan;117(1):33-8. Vanillin and furfural are derived from lignocellulosic biomass and inhibit yeast growth and fermentation as biomass conversion inhibitors. Furfural has been shown to induce oxidative stress in Saccharomyces cerevisiae. |
Preparing Stock Solutions of Vanillin
| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 6.5729 mL | 32.8645 mL | 65.7289 mL | 131.4579 mL | 164.3223 mL |
| 5 mM | 1.3146 mL | 6.5729 mL | 13.1458 mL | 26.2916 mL | 32.8645 mL |
| 10 mM | 0.6573 mL | 3.2864 mL | 6.5729 mL | 13.1458 mL | 16.4322 mL |
| 50 mM | 0.1315 mL | 0.6573 mL | 1.3146 mL | 2.6292 mL | 3.2864 mL |
| 100 mM | 0.0657 mL | 0.3286 mL | 0.6573 mL | 1.3146 mL | 1.6432 mL |
| * Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. | |||||
References on Vanillin
Vanillin protects human keratinocyte stem cells against ultraviolet B irradiation.[Pubmed:24184596]
Food Chem Toxicol. 2014 Jan;63:30-7.
Ultraviolet-B (UVB) irradiation is one of major factors which induce cellular damages in the epidermis. We investigated protective effects and mechanisms of Vanillin, a main constituent of vanilla beans, against UVB-induced cellular damages in keratinocyte stem cells (KSC). Here, Vanillin significantly attenuated UVB irradiation-induced cytotoxicity. The Vanillin effects were also demonstrated by the results of the senescence-associated beta-galactosidase and alkaline comet assays. In addition, Vanillin induced production of pro-inflammatory cytokines. Attempts to elucidate a possible mechanism underlying the Vanillin-mediated effects revealed that Vanillin significantly reduced UVB-induced phosphorylation of ataxia telangiectasia mutated (ATM), serine threonine kinase checkpoint kinase 2 (Chk2), tumor suppressor protein 53 (p53), p38/mitogen-activated protein kinase (p38), c-Jun N-terminal kinase/stress-activated protein kinase (JNK), S6 ribosomal protein (S6RP), and histone 2A family member X (H2A.X). UVB-induced activation of p53 luciferase reporter was also significantly inhibited by Vanillin. In addition, while ATM inhibitor had no effect on the Vanillin effects, mouse double minute 2 homolog (MDM2) inhibitor significantly attenuated suppressive effects of Vanillin on UVB-induced activation of p53 reporter in KSC. Taken together, these findings suggest that Vanillin protects KSC from UVB irradiation and its effects may occur through the suppression of downstream step of MDM2 in UVB irradiation-induced p53 activation.
Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass.[Pubmed:24997375]
Bioresour Technol. 2014 Sep;167:324-30.
Pretreatment of lignocellulosic material produces a wide variety of inhibitory compounds, which strongly inhibit the following enzymatic hydrolysis of cellulosic biomass. Vanillin is a kind of phenolics derived from degradation of lignin. The effect of Vanillin on cellulase activity for the hydrolysis of cellulose was investigated in detail. The results clearly showed that Vanillin can reversibly and non-competitively inhibit the cellulase activity at appropriate concentrations and the value of IC50 was estimated to be 30 g/L. The inhibition kinetics of cellulase by Vanillin was studied using HCH-1 model and inhibition constants were determined. Moreover, investigation of three compounds with similar structure of Vanillin on cellulase activity demonstrated that aldehyde group and phenolic hydroxyl groups of Vanillin had inhibitory effect on cellulase. These results provide valuable and detailed information for understanding the inhibition of lignin derived phenolics on cellulase.
Importance of glucose-6-phosphate dehydrogenase (G6PDH) for vanillin tolerance in Saccharomyces cerevisiae.[Pubmed:24725964]
J Biosci Bioeng. 2014 Sep;118(3):263-9.
Vanillin is derived from lignocellulosic biomass and, as one of the major biomass conversion inhibitors, inhibits yeast growth and fermentation. Vanillin was recently shown to induce the mitochondrial fragmentation and formation of mRNP granules such as processing bodies and stress granules in Saccharomyces cerevisiae. Furfural, another major biomass conversion inhibitor, also induces oxidative stress and is reduced in an NAD(P)H-dependent manner to its less toxic alcohol derivative. Therefore, the pentose phosphate pathway (PPP), through which most NADPH is generated, plays a role in tolerance to furfural. Although Vanillin also induces oxidative stress and is reduced to vanillyl alcohol in a NADPH-dependent manner, the relationship between Vanillin and PPP has not yet been investigated. In the present study, we examined the importance of glucose-6-phosphate dehydrogenase (G6PDH), which catalyzes the rate-limiting NADPH-producing step in PPP, for yeast tolerance to Vanillin. The growth of the null mutant of G6PDH gene (zwf1Delta) was delayed in the presence of Vanillin, and Vanillin was efficiently reduced in the culture of wild-type cells but not in the culture of zwf1Delta cells. Furthermore, zwf1Delta cells easily induced the activation of Yap1, an oxidative stress responsive transcription factor, mitochondrial fragmentation, and P-body formation with the Vanillin treatment, which indicated that zwf1Delta cells were more susceptible to Vanillin than wild type cells. These findings suggest the importance of G6PDH and PPP in the response of yeast to Vanillin.
Vanillin causes the activation of Yap1 and mitochondrial fragmentation in Saccharomyces cerevisiae.[Pubmed:23850265]
J Biosci Bioeng. 2014 Jan;117(1):33-8.
Vanillin and furfural are derived from lignocellulosic biomass and inhibit yeast growth and fermentation as biomass conversion inhibitors. Furfural has been shown to induce oxidative stress in Saccharomyces cerevisiae. Since there has been no report on the relationship between Vanillin and oxidative stress, we investigated whether Vanillin caused oxidative stress in yeast cells. We showed that Vanillin caused the nuclear accumulation of Yap1, an oxidative stress responsive transcription factor, and subsequent transcriptional activation of Yap1-target genes. The growth of the null mutant of the YAP1 gene (yap1Delta) was delayed in the presence of Vanillin, which indicated that Yap1 plays a role in the acquisition of tolerance to Vanillin. We also demonstrated that Vanillin facilitated the fragmentation of mitochondria. These findings suggest that the toxicity of Vanillin involves damage induced by oxidative stress.
Description
Vanillin (p-Vanillin) is a single molecule extracted from vanilla beans and also a popular odor used widely in perfume, food and medicine.
Keywords:
Vanillin,121-33-5,4-Hydroxy 3-methoxybenzaldehyde; Vanillic aldehyde,Natural Products,Others, buy Vanillin , Vanillin supplier , purchase Vanillin , Vanillin cost , Vanillin manufacturer , order Vanillin , high purity Vanillin
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