2-Ketoglutaric acid

Details:

About:
α-ketoglutaric acid is an important biomolecule, one of the important intermediates in the tricarboxylic acid cycle, a nitrogen transporter and a symbiotic substance in molecular oxidation. It plays an important role in the metabolism of microbial cells, and is also an important precursor for the synthesis of a variety of amino acids and proteins.

Physiological effects:
α-ketoglutaric acid is a kind of short-chain carboxylic acid with multiple functions, which does not show toxicity and has high stability in aqueous solution. In vivo, AKG is not only an important transitional product in the TCA cycle, but also a precursor of L-glutamic acid, L-glutamine, L-proline and L-arginine. Experimental studies on animals have shown that AKG, glutamine and glutamate ingested by animals are absorbed and metabolized in the upper part of the small intestine, in which up to 95% of glutamate and 70% of glutamine are degraded into CO2, while only 40% of AKG is degraded, and the remaining AKG can be used for the metabolic process of intestinal cells and peripheral tissues.

Preparation:
The steps of bioconversion to synthesize alpha-ketoglutaric acid are as follows:
(1) The bacteria of Max Kluyveria ATCC36534 stored in the refrigerator were inoculated on fresh bevel medium and cultured in a constant temperature incubator at 25 ~ 30℃ for 24 ~ 36h to obtain activated Max Kluyveria ATCC36534 strain. The incline medium consists of glucose 10 ~ 20g/L, peptone 5 ~ 10g/L, yeast leaching powder 3 ~ 5g/L, AGAR 15 ~ 20g/L, water as solvent, natural pH (measured 6.3 ~ 6.5), high pressure steam 121℃ sterilization 15 ~ 20min.
(2) The 2 ~ 3 rings of the slant strains of Max Kluyveromycetes ATCC36534 after activation culture were selected with the inoculation ring step (1) and inoculated into the seed medium. The seed culture after inoculation was based on 20-24h culture at 25 ~ 30℃ and 200 ~ 250r/min oscillating conditions, and the seed solution with a dry cell concentration of 5 ~ 7g/L was obtained. The seed medium composition is the same as the inclined medium in step (1), except without AGAR, and the filling amount in the triangular bottle is 20% ~ 40% of its volume. The triangular bottle is sealed with 8 layers and sterilized by high pressure steam at 121℃ for 15 ~ 20min.
(3) Select the 3 ~ 5 rings of Max Kluyveria ATCC36534 inclined surface bacteria after activation culture in step (1), or the seed solution prepared in step (2), and add 5% ~ 10% of the volume to the transformation medium, and culture at 25 ~ 30℃ and 200 ~ 250r/min for 20 ~ 24h. The dry cell concentration was 6-10g /L, and the final concentration was 10-50mmol /L H2O2, or the final concentration was 0.5-1.0 μmol/L methotrexate, or both were added at the same time. Then L-glutamic acid with a final concentration of 20 to 50g/L was added. Under the same conditions, the synthetic culture medium of α-ketoglutaric acid with the concentration of 17.3 ~ 42.9g/L was obtained in triangle flask for 20 ~ 24h. The composition of the conversion medium is: Sucrose 50 ~ 100g/L, yeast leaching powder 10 ~ 20g/L, KH2PO43 ~ 5g/L, K2HPO45 ~ 6.5g/L, MgSO40.5 ~ 1.0g/L, the solvent is tap water, the pH is natural (measured 6.5), the amount in the triangle bottle is 20% ~ 40% of its volume. The triangular bottle was sealed with 8 layers and sterilized by high pressure steam at 121℃ for 15 ~ 20min.
(4) The synthetic culture medium prepared in step (3) was centrifuged for 3000 ~ 4000g and 5 ~ 10min to remove bacteria, then supernatant a was obtained, activated carbon was added, and activated carbon was filtered after stirring for 30 ~ 60min. The filtrate was reduced pressure and concentrated to 1/2 ~ 1/4 of the original volume, and the sediment was removed again by centrifugation to obtain supernatant b. Add α-ketoglutaric acid seeds, slowly stir and cool to 4℃, stand for 8 ~ 12h, filter and collect α-ketoglutaric acid crystals, vacuum drying at 45℃ to obtain crystalline α-ketoglutaric acid; The volume of the activated carbon added above is 5 ~ 10g/L, and the volume of the α-ketoglutaric acid added above is 10 ~ 20g/L

Company Introduction

hebei yanxi chemical  co., LTD who registered capital of 10 million yuan, nearly to $2 million, we have a pharmaceutical raw materials factory production of pharmaceutical raw materials, and a reagent r&d center, and we do research and development production of reagent tens of thousands of species. our aim is quality strives for the survival. Seek development by credit reputation. Our products have big price advantage in Europe South America and so on .and Our company also has the advantage of high quality in Africa. in the past two years our products has reached more than 30 countries in the world, Europe. South America. North America, southeast Asia, Africa, etc. We welcome all over the world with the best quality and lowest price friend common cooperation and common development!

Our advantages

1) Timely communication, reply within 12 hours.You can leave any messages on our whatsapp or send email to us.

2)Selecting raw materials strictly,our products are the highest purity.

3)Reasonable and competitive price.

4) Free sample for your evaluation.Customers only need to undertake the shipping fee for delivery the samples to your destionation.

5)OEM/ODM Available.Trial order is available for testing after samples.

6) Packaging can be customized according to each buyers' special request.

We guarantee that your package will pass 100% through the Mexico US, UK, Australia, Netherlands, Canada, France, Germany, Spain, Belgium, Sweden, Poland, Russian, Ukraine, Kazakhstan, Czech Republic, Slovakia, Portugal.

We have freight forwarders in these countries, and our customs clearance company will clear your parcels without any customs issues, sometimes even 1000 kg. Door to door service. Make sure you receive 100% of the goods. Let you have a good experience of our strong freight capacity.

After Sales Service

1) Best quality in your requirement

2)Competitive price in china market

3)Mature technical support

4)Professional logistic support

5 )Full experience of large numberscontainers loading in chinese sea port

6 )Fast shipment by reputed shipping line

7) Packing with pallet as buyer's special request

8) Best service after shipment.

9) Cargoes together with container after-sales service available

10) Full experience in export

11)Raw materials from chinese origin

FAQ

1.What is the way you ship

Express: FedEx, UPS, EMS, DHL, USPS, TNT, DPD, Aramex etc

Our way of shipping the products is universal and secure.

The company name and other contents related to pharmacy are not written on the package slips.

The way of packing is regularly changed for a secure delivery.

The packages are small in order to avoid unwelcomed questions . If the order is big, then it will be divided into two or more packages

2.Can the packages be trackable

YES, we provide tracking number and you can track on internet.

3.Where do you ship to

Worldwide.

4.Do you offer domestic shipping/remail shipping

Yes we can ship to USA/UK/Canada/Australia/Germany etc warehouse first then to you.

5.Do you ensure your packing, what about customs, what happens if the package is retained

We concern a lot about our safety system, we pack the orders as secure as possible. If a package is seized at customs, reships of seized orders will be done provided you have a copy of the letter to show us. If you receive a seizure letter, please contact us immediately with a scanned copy of the seizure letter, and also a new address to have your order reshipped to.

6.Which payment options are available

Bitcoin,,Bank wire,MoneyGram