(Z-DEVD)2-Rhodamine 110 4,4'-[9-(2-Carboxyphenyl)xanthylium-3,6-diyl]bis[N-[(phenylmethoxy)carbonyl]-L-alpha-aspartyl-L-alpha-glutamyl-L-valyl-L-alpha-asparagine inner salt
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(Z-Asp-Glu-Val-Asp)2-Rhodamine 110, a highly sensitive and photostable fluorogenic substrate, is used to determine intracellular caspase-3 activity. Fluorophore Rhodamine 110 (R110) can be detected at an emission wavelength of 535 nm using an excitation wavelength of 485 nm. As with (H-Asp-Glu-Val-Asp)2-Rhodamine 110, the fluorescence intensity can be measured by flow cytometry, microwell plate reader, or fluorescence microscopy.
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