DNase II

Details:

CAS number: 9025-64-3
Level: BR
Vitality: ≥ 800 units/mg dry weight  
Definition of vitality: One Unit causes an increase in absorption at 260 nm of 0.001 per minute at 25 ° C, pH 4.6 using highly polymerized DNA as substrate.
Appearance: White or off white freeze-dried powder. It is a double stranded specific endonuclease that can degrade double stranded DNA into oligodeoxyribonucleotides with 5 '- phosphate and 3' - OH termini. When monovalent manganese ions are present, they can also degrade single stranded DNA. Dissolved in water, slightly soluble in 30% ethanol and acetone. Enzyme reaction: Deoxyribonucleic acid → Dinucleotide-5 '- Phosphate+Oligonucleotide 5' - Phosphate, with an optimal pH of 7.8. Absorption coefficient (E1% 280) 11.1. The activator is a divalent metal ion, and the maximum activation can be obtained from magnesium (Mg2+)+calcium (Ca2+). Inhibitors include EDTA and sodium dodecyl sulfate. Can add 5mmol/L calcium (Ca2+)
Purpose: Biochemical research. Used for molecular biology research. Various enzymes that degrade DNA. These enzymes hydrate the phosphodiester bonds on the sugar phosphate backbone
Solution. There are two types of enzymes in this category: exonucleases and endonucleases. There are many types of DNAases, some are single stranded specific and some are double stranded specific
Sexual or sequence specific (such as restriction endonucleases). Restriction endonucleases have the ability to recognize specific base sequences, which has been used in gene manipulation techniques to construct artificial clones. This product is only for scientific research use
Storage: -20 ℃

Nanjing Duly Biotech Co.,Ltd 

has been established for 11 years. It  is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.

Our company’s aim is to provide customers with the best quality products and the most intimate service.Personnel has been trained; every batch of material is checked; each procedure guarantees the quality; every bag of our products is top grade;service is thoughtful all the time.The quality, colors and forms of our products have reached a high level in related chemical industry, which relies on characteristic and advanced processing technology and it has gained a high and reliable reputation from customers at both quality and services.

FAQ

Q1: Are you a manufacturer?

Answer: Yes, we are factory founded on 2009.

Q2:How to contact with us?

Answer: Search our company name as Nanjing Duly Biotech Co., Ltd. Please contact sales for specific information.

Q3:Can you give me a discount price?

Answer:Surely,It depend on your qty.

Q4: How can i get a sample?

Answer:Free sample is available,but freight charges will be at your account and the charges will be return to you dudect from your order in future.

Q5:What are the main categories of products sold by the company?

Answer: It  is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, antibiotics, plant hormones, nucleic acids, pigments, amino acids, vitamins, carbohydrates, buffers, surfactants, culture media and Separation of materials and kits.

Q6:Is the company mainly engaged in small packaging or large packaging?

Answer: Mainly do small packaging reagents, large packaging also do, specific can ask customer service personnel.